Complete Genome Sequence of Methylosinus sp. Strain C49, a Methane-Oxidizing Bacterium Harboring phaABC Genes for Polyhydroxyalkanoate Synthesis

Abstract

We report a complete genome sequence of Methylosinus sp. strain C49, a methane-oxidizing bacterium (MOB) in the class Alphaproteobacteria, isolated from MOB-enriched biomass. The genome encodes the functional genes for methane oxidation (pmoA) and polyhydroxyalkanoate (PHA) biosynthesis (phaABC). Deciphering the genome will help research toward PHA production by MOB.

FIG 1

Methane oxidation activity of Methylosinus sp. strain C49 during batch culture. Solid circle, square, and triangle plots indicate methane, oxygen, and carbon dioxide concentrations, respectively, in the headspace. A control run without the cell suspension was operated in parallel to confirm the absence of unintentional methane consumption. Open circle, square, and triangle plots indicate methane, oxygen, and carbon dioxide concentrations, respectively, in the headspace. The experiment was conducted in triplicate.