Nearly Complete Genome Sequence of Brugia pahangi FR3

Abstract

Brugia pahangi is a zoonotic parasite that is closely related to human-infecting filarial nematodes. Here, we report the nearly complete genome of Brugia pahangi, including assemblies of four autosomes and an X chromosome, with only seven gaps. The Y chromosome is still not completely assembled.

FIG 1

Synteny between Brugia pahangi and Brugia malayi chromosomes. The current Brugia pahangi genome was aligned to the Brugia malayi V4 genome (7) using NUCmer v3.1 (default options). The resulting delta file was converted into tabular form using the show-coords function with the option -qlTHb. Contigs >4.5% of the length of the matching B. malayi chromosome were identified in R (v. 3.4.4 [9]; libraries: RColorBrewer, ggplot2, data.table) and plotted with ggplots by chromosome. (A) Chromosome 1; (B) chromosome 2; (C) chromosome 3; (D) chromosome 4; (E) chromosome X. This nearly complete B. pahangi genome spans most of the B. malayi genome with some rearrangements. The orthologous regions of the genomes vary in percent identity but typically show >90% identity, with more nucleotide variation at the ends of chromosomes, relative to the middle of the same chromosomes, using the B. malayi chromosomes as references.