Deinococcus detaillensis sp. nov., isolated from humus soil in Antarctica

Abstract

A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1^T, was isolated from a humus soil sample in the Detaille Island of Antarctica. The 16S rRNA gene sequence result indicated that strain H1^T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%). Growth of strain H1^T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v). The respiratory quinone was MK-8. The major fatty acids were C_16:0, C_17:0 cyclo and summed feature 3 (C_16:1 ω7c/C_16:1 ω6c). The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid. The cell wall peptidoglycan type was A3β. The genomic DNA G + C content was 61.3 mol%. The average nucleotide identity (ANI) between strain H1^T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification. Based on the above results, strain H1^T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp. nov. is proposed. Type strain is H1^T (= CGMCC 1.13938^T = JCM 33291^T).

Keywords: Deinococcus detaillensis; Humus soil; Polyphasic taxonomy.