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Review
. 2020 Aug:57:75-81.
doi: 10.1016/j.cbpa.2020.04.020. Epub 2020 Jun 30.

Advances of epigenetic editing

Rutger A F Gjaltema  1 , Marianne G Rots  2
Affiliations
Free article
Review

Advances of epigenetic editing

Rutger A F Gjaltema et al. Curr Opin Chem Biol. 2020 Aug.
Free article

Abstract

Epigenetic editing refers to the locus-specific targeting of epigenetic enzymes to rewrite the local epigenetic landscape of an endogenous genomic site, often with the aim of transcriptional reprogramming. Implementing clustered regularly interspaced short palindromic repeat-dCas9 greatly accelerated the advancement of epigenetic editing, yielding preclinical therapeutic successes using a variety of epigenetic enzymes. Here, we review the current applications of these epigenetic editing tools in mammals and shed light on biochemical improvements that facilitate versatile applications.

Keywords: CRISPR/dCas; Epigenome editing; Expression reprogramming; Targeted DNA methylation; Targeted histone modifications.

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Conflict of interest statement

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: M.G.R. reports serving as a consultant to Sangamo Therapeutics, Richmond, CA.

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