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. 1988;27(2):103-8.
doi: 10.1007/BF00200012.

Species-specific TNF induction of thymocyte proliferation

M J Ehrke  1 , R L Ho, K Hori
Affiliations

Species-specific TNF induction of thymocyte proliferation

M J Ehrke et al. Cancer Immunol Immunother. 1988.

Abstract

Recombinant murine (rMu) tumor necrosis factor (TNF), in a standard comitogenic assay with phytohemagglutinin, induced murine thymocyte proliferation, while up to 10,000-fold higher concentrations of recombinant human TNF did not. The induction of thymocyte proliferation was dependent upon TNF concentration in a biphasic manner. Thus, 100 to 1,000 units/ml TNF were near optimal while concentrations greater than or equal to 1,000 units/ml caused apparent down regulation. The effect was abrogated by neutralizing antibody to rMu-TNF but not by neutralizing antibody to rMu-interleukin 1 alpha or beta. The rMu-TNF did not induce proliferation of the mature murine T-helper cell line, D10.G4.1, in the presence of mitogen. Taken together the results indicate that TNF, in a strictly species-specific manner, can regulate thymocyte proliferation independently of interleukin 1.

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