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. 1988;27(2):103-8.
doi: 10.1007/BF00200012.

Species-specific TNF induction of thymocyte proliferation

M J Ehrke  1 , R L Ho, K Hori
Affiliations

Species-specific TNF induction of thymocyte proliferation

M J Ehrke et al. Cancer Immunol Immunother. 1988.

Abstract

Recombinant murine (rMu) tumor necrosis factor (TNF), in a standard comitogenic assay with phytohemagglutinin, induced murine thymocyte proliferation, while up to 10,000-fold higher concentrations of recombinant human TNF did not. The induction of thymocyte proliferation was dependent upon TNF concentration in a biphasic manner. Thus, 100 to 1,000 units/ml TNF were near optimal while concentrations greater than or equal to 1,000 units/ml caused apparent down regulation. The effect was abrogated by neutralizing antibody to rMu-TNF but not by neutralizing antibody to rMu-interleukin 1 alpha or beta. The rMu-TNF did not induce proliferation of the mature murine T-helper cell line, D10.G4.1, in the presence of mitogen. Taken together the results indicate that TNF, in a strictly species-specific manner, can regulate thymocyte proliferation independently of interleukin 1.

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References

    1. Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA. 1975;72:3666–3670. - PMC - PubMed
    1. Elias JA, Gustilo K, Baeder W, Freundlich B. Synergistic stimulation of fibroblast prostaglandin production by recombinant interleukin 1 and tumor necrosis factor. J Immunol. 1987;138:3812–3816. - PubMed
    1. Fransen L, Ruysschaert MR, Van Der Heyden J, Fiers W. Recombinant tumor necrosis factor: Species specificity for a variety of human and murine transformed cell lines. Cell Immunol. 1986;100:260–267. - PubMed
    1. Hori K, Ehrke MJ, Mace K, Mihich E. Effect of recombinant tumor necrosis factor on tumoricidal activation of murine macrophages: Synergism between tumor necrosis factor and γ-interferon. Cancer Res. 1987;47:5868–5874. - PubMed
    1. Huang JJ, Newton RC, Rutledge SJ, Horuk R, Matthew JB, Covington M, Lin Y. Characterization of murine IL-1β, isolation, expression and purification. J Immunol. 1988;140:3838–3843. - PubMed

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