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. 2020 Jun;75(2):267-274.
doi: 10.22092/ari.2018.120218.1186. Epub 2020 Jun 1.

Molecular and Serological Study of Neospora caninum Infection among Dogs and Foxes in Sanandaj, Kurdistan Province, Iran

Gh Adhami  1 , A Dalimi  2   2 , N Hoghooghi-Rad  1 , Sh Fakour  3
Affiliations

Molecular and Serological Study of Neospora caninum Infection among Dogs and Foxes in Sanandaj, Kurdistan Province, Iran

Gh Adhami et al. Arch Razi Inst. 2020 Jun.

Abstract

Neospora caninum, a protozoan parasite, causes abortions in cattle, as well as neurological disorders and reproductive problems in dogs. This study aimed to investigate the serological and the molecular prevalence of N.caninum among foxes and dogs using indirect fluorescent antibody test (IFAT) and nested-polymerase chain reaction (PCR). For this purpose, 288 and 95 both fecal and serum samples of dogs and foxes were collected, respectively, from around industrial and traditional dairy flocks in different parts of Sanandaj, Kurdistan Province, Iran, from 2013 to 2015. The sera were examined using IFAT, and fecal samples were microscopically assessed for detecting Neospora oocyst and by nested-PCR. The findings revealed that N.caninum seroprevalence were 4.86% and 4.21% in dogs and foxes, respectively. In addition, no Neospora oocysts were found microscopically and by PCR. Since this study is the first serological and molecular investigation of N.caninum among both dogs and foxes in Sanandaj, the findings of indicated that stray dogs is a main source of N.caninum infection in dairy farms in Sanandaj, Iran.

Keywords: Dog; Fox; Indirect Fluorescent Antibody Test (IFAT); Iran; Neospora Caninum; Nested- polymerase chain reaction (PCR); Sanandaj.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
The geographic location of the study area (Sanandaj district, Kurdistan Province, Iran)
See this image and copyright information in PMC

References

    1. Abdoli A, Arbabi M, Dalimi A, Pirestani M. Molecular detection of Neospora caninum in house sparrows (Passer domesticus) in Iran. Avian Pathol. 2015;44(4):319–22. - PubMed
    1. Acosta ID, Centoducatte LD, Soares HS, Marcili A, Gondim MF, Rossi JLJ, et al. Occurrence of Neospora caninum and Toxoplasma gondii antibodies in dogs from rural properties surrounding a biological reserve, Espirito Santo, Brasil. Rev Bras Parasitol Vet. 2016;25:536–539. - PubMed
    1. Bahrami S, Boroomand Z, Alborzi AR, Namavari M, Mousavi SB. A molecular and serological study of Neospora caninum infection in pigeons from southwest Iran. Vet Arh. 2016;86:815–23.
    1. Bartley PM, Wright SE, Zimmer IA, Roy S, Kitchener AC, Meredith A, Innes EA, Katzer F. Detection of Neospora caninum in wild carnivorans in Great Britain. Vet Parasitol. 2013;192(1-3):279–83. - PubMed
    1. Coskun TH, Grandpierre AG, Christodoulides DN, Segev M. Coherence enhancement of spatially incoherent light beams through soliton interactions. Opt Lett. 2000; 25: 826–828. - PubMed

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