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Comment
. 2021 Jan;82(1):159-198.
doi: 10.1016/j.jinf.2020.06.075. Epub 2020 Jul 2.

The determination of release from isolation of COVID-19 patients requires ultra-high sensitivity nucleic acid test technology

Wanqiu Huang  1 , Dachuan Lin  2 , Cuini Wang  1 , Chaohui Bao  1 , Zhaoqi Zhang  3 , Xinchun Chen  4 , Zheng Zhang  5 , Jian Huang  6
Affiliations
Comment

The determination of release from isolation of COVID-19 patients requires ultra-high sensitivity nucleic acid test technology

Wanqiu Huang et al. J Infect. 2021 Jan.
No abstract available

Keywords: COVID-19; False negative; Nucleic acid detection; SARS-CoV-2; nestRPA.

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Conflict of interest statement

Declaration of Competing Interest No authors declared any potential conflicts of interest.

Figures

Fig. 1
Fig. 1
Nucleic acid detection results using nestRPA. (A) The distribution of target fragments on SARS-CoV-2 genome. (B) The LOD of optimum primer pairs from different gene regions. (C) The sensitivity of outer primers for Fragment 5. (D) The sensitivity of inner primers for Fragment 5. (E) The sensitivity of outer primers for Fragment 7. (F) The sensitivity of inner primers for Fragment 7. (G) The sensitivity of nestRPA for Fragment 5. (H) The sensitivity of nestRPA for Fragment 7. (I) The five positive results of four people returning to work by nestRPA. (J) Statistics of nucleic acid detection results by nestRPA and qPCR assays for SARS-CoV-2. “*”, the statistical difference of fluorescence intensity difference between test sample and blank control serves as the criterion for judging the positive (p < 0.05) of per reaction.
Fig. 2
Fig. 2
Comparison of clinical sampling method and a protective sampling kit with light source. (Left) Wrong sampling method; (Middle) Correct sampling method; (Right) protective sampling kit with light source. This device is a protective oral-nasopharyngeal sampling set with built-in light source, including 7 components: (1) LED inspection lamp handle; (2) LED inspection light; (3) Disposable use of anti-droplet baffle; (4) U-shaped slot; (5) Sterile swab; (6) Sampling hole; (7) Sterile tongue spatula.
See this image and copyright information in PMC

Comment on

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