Improvement in estimation of time since death by albumin and potassium concentrations in vitreous humor

Abstract

The measurement of the potassium concentration (K⁺) in vitreous humor (VH) has been a well-known adjunct for the estimation of the post-mortem interval (PMI) since the early 1960s. For years, however, many authors have been using other biochemical markers in an attempt to improve predictions. In this paper we confirm the role of K⁺ in the determination of the PMI adopting a linear regression model and we investigate whether other biochemical markers could improve the model through a multiple regression analysis. Additionally, the research aims to confirm the data of the analytes of interest among different techniques and instrumentations. We deemed this as an important issue because a primary concern in the literature is that automated analytical methods are often calibrated and, for the most part, validated for serum or urine analysis. Our results confirmed the well-established role of K⁺ as well as highlighted Albumin as a novel marker to be considered for further improvement of prediction models, especially since 72 h after death.

Keywords: Albumin; Post-mortem interval; Potassium; Regression analysis; Vitreous humor.