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. 2018 Dec 20;19(3):168-174.
doi: 10.1002/elsc.201800095. eCollection 2019 Mar.

Salicylic acid-enhanced biosynthesis of pharmacologically important lignans and neo lignans in cell suspension culture of Linum ussitatsimum L

Muhammad Nadeem  1 , Waqar Ahmad  1 , Adnan Zahir  1 , Christophe Hano  2 , Bilal Haider Abbasi  1   2   3
Affiliations

Salicylic acid-enhanced biosynthesis of pharmacologically important lignans and neo lignans in cell suspension culture of Linum ussitatsimum L

Muhammad Nadeem et al. Eng Life Sci. .

Abstract

Linum usitatsimum L. (flax) is a perennial herb with magnitude of medicinal and commercial applications. In the present study, we investigated the effects of salicylic acid (SA) on biosynthesis of lignans (secoisolariciresinol diglucoside (SDG) and lariciresinol diglucoside (LDG)) and neolignans (dehydrodiconiferyl alcohol glucoside (DCG) and guaiacylglycerol-β-coniferyl alcohol ether glucoside (GGCG)) in cell cultures of flax. Moderate concentration of SA (50 μM) enhanced biomass accumulation (10.98 g/L dry weight (DW)), total phenolic content (37.81 mg/g DW), and antioxidant potential (87.23%) to two-fold than their respective controls after 72 h of exposure. However, higher levels of total flavonoid content (5.32 mg/g DW) were noted after 48 h of exposure to 50 μM of SA. HPLC analyses revealed that 50 μM SA, significantly enhanced biosynthesis of SDG (7.95 mg/g DW), LDG (7.52 mg/g DW), DCG (54.90 mg/g DW), and GGCG (16.78 mg/g DW), which was almost 2.7, 1.8, 3.88, and 3.98 fold higher than their respective controls after 72 h of exposure time, respectively. These results indicated that moderate concentrations of SA had significant effects on biosynthesis and productivity of lignans and neolignans in cell culture of L. usitatissimum.

Keywords: HPLC; Linum usitatissimum; antioxidant; lignans; neo‐lignans; salicylic acid.

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Conflict of interest statement

The authors have declared no conflict of interest.

Figures

Figure 1
Figure 1
Time coruse growth of biomass and polyphenol accumulation in cell cultues of flax. Values indicates mean ± SE
Figure 2
Figure 2
Effect of SA on accumulation of biomass DW in flax cell cultures. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05)
Figure 3
Figure 3
(A) Effect of SA on biosynthesis of TPC in flax cell cultures. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05). (B) Effect of SA on biosynthesis of TFC in flax cell cultures. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05)
Figure 4
Figure 4
Effect of SA on antioxidant potetnial of flax cell cultures. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05)
Figure 5
Figure 5
(A) Effect of SA on SDG biosynthesis in cell cultures of flax. Columns with identical letters are not significantly different (P < 0.05). (B) Effect of SA on LDG biosynthesis in cell cultures of flax. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05). (C) Effect of SA on DCG biosynthesis in cell cultures of flax. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05). (D) Effect of SA onGGCG biosynthesis in cell cultures of flax. Values indicates mean ± SE. Columns with identical letters are not significantly different (P < 0.05)
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