Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jul 6;18(1):19.
doi: 10.1186/s43141-020-00033-x.

Pterocarpus marsupium Roxb. heartwood extract synthesized chitosan nanoparticles and its biomedical applications

Anupama Ammulu Manne  1 , Vinay Viswanath K  1 , Ajay Kumar G  1 , Ushakiranmayi Mangamuri  2 , Sudhakar Podha  3
Affiliations

Pterocarpus marsupium Roxb. heartwood extract synthesized chitosan nanoparticles and its biomedical applications

Anupama Ammulu Manne et al. J Genet Eng Biotechnol. .

Abstract

Background: The point of the present investigation was to blend effective chitosan nanoparticles (CNPs) loaded with Pterocarpus marsupium (PM) heartwood extract and evaluate its biomedical applications. Various plant extract concentrations (PM-CNPs-1, PM-CNPs-2, PM-CNPs-3) are used to synthesize chitosan nanoparticles and optimized to acquire a stable nanoparticle formulation. The entrapment efficiency and in vitro release studies of the plant extract encapsulated in CNPs are estimated. The PM-loaded CNPs were characterized by X-ray diffraction, dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The synthesized chitosan nanoparticles were evaluated for their alpha-amylase inhibitory activity and inhibition of albumin denaturation activity.

Results: The XRD pattern of PM-CNPs shows less number of peaks at low intensity due to the interaction of chitosan with sodium tripolyphosphate. The FT-IR spectrum with peaks at 1639.55 and 1149.02 cm-1 confirms the formation of chitosan nanoparticles. The size of the nanoparticles ranges between 100 and 110 nm with spherical shape illustrated by SEM and TEM analysis. The nanoparticle formulation with 10% plant extract concentration (PM-CNPs-2) showed optimum particle size, higher stability, enhanced entrapment efficiency, and sustained drug release characteristics. Synthesized chitosan nanoparticles have shown a significant increase in alpha-amylase inhibition and appreciable anti-inflammatory activity as measured by inhibition of protein denaturation.

Conclusions: The investigation reports the eco-friendly, cost-effective method for synthesizing chitosan nanoparticles loaded with Pterocarpus marsupium Rox.b heartwood extract.

Keywords: Alpha-amylase inhibitory activity; Anti-inflammatory activity; Chitosan nanoparticles; Pterocarpus marsupium.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
XRD pattern of chitosan nanoparticles synthesized by Pterocarpus marsupium Rox.b heartwood extract
Fig. 2
Fig. 2
Zeta potential distribution of chitosan nanoparticles (CNPs) loaded with Pterocarpus marsupium (PM) stem bark extract concentrations. a CNPs. b PM-CNPs-1. c PM-CNPs-2. d PM-CNPs-3
Fig. 3
Fig. 3
Particle size distribution of chitosan nanoparticles (CNPs) loaded with different P. marsupium stem bark extract concentrations. a PM-CNPs-1. b PM-CNPs-2. c PM-CNPs-3
Fig. 4
Fig. 4
FT-IR spectrums of aPterocarpus marsupium (PM) aqueous stem bark extract, b chitosan nanoparticles (CNPs), and cPterocarpus marsupium-loaded chitosan nanoparticles (PM-CNPs)
Fig. 5
Fig. 5
SEM micrographs of a chitosan nanoparticles at 10 μm. bPterocarpus marsupium extract-loaded chitosan nanoparticles at 10 μm scale
Fig. 6
Fig. 6
TEM micrograph of Pterocarpus marsupium-loaded chitosan nanoparticles at 100 nm magnification
Fig. 7
Fig. 7
Drug Release profile of Pterocarpus marsupium extract-loaded chitosan nanoparticles
Fig. 8
Fig. 8
Representing alpha-amylase inhibition evaluated by treating with various concentrations of PM-CNPs-2 and Acarbose
Fig. 9
Fig. 9
Albumin inhibitory activity evaluated by treating with various concentrations of PM-CNPs-2 and diclofenac sodium
See this image and copyright information in PMC

References

    1. Colvin VL, Schlamp MC, Alivisatos A. Light-emitting diodes made from cadmium selenide nanocrystals and a semiconducting polymer. Nature. 1994;370:354–357. doi: 10.1038/370354a0. - DOI
    1. Wang Y, Herron N. Nanometer-sized semiconductor clusters: Materials synthesis, quantum size effects, and photophysical properties. J Phys Chem. 1991;95:525–532. doi: 10.1021/j100155a009. - DOI
    1. Kreuter J. Nanoparticulate systems for brain delivery of drugs. J Adv Drug Delivery Reviews. 2001;47:65–81. doi: 10.1016/s0169-409x(00)00122-8. - DOI - PubMed
    1. Jafarizadeh-Malmiri H, Gaz-Jahanian MA, Berenjian A. Potential applications of Chitosan nanoparticles as novel support in enzyme immobilization. Am J Biochem Biotechnol. 2012;8:203–219. doi: 10.3844/ajbbsp.2012.203.219. - DOI
    1. Schmid G. Large clusters and colloids. Metals in the embryonic state. Chem Rev. 1992;92:1709–1727. doi: 10.1021/cr00016a002. - DOI

LinkOut - more resources