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. 2021 Jan;76(1):366-371.
doi: 10.1111/all.14486. Epub 2020 Jul 17.

RNA-seq-based profiling of extracellular vesicles in plasma reveals a potential role of miR-122-5p in asthma

Thomas Bahmer  1   2 , Susanne Krauss-Etschmann  2   3 , Dominik Buschmann  4 , Jochen Behrends  5 , Henrik Watz  6 , Anne-Marie Kirsten  6 , Frauke Pedersen  1   6 , Benjamin Waschki  1   7 , Oliver Fuchs  8   9 , Michael W Pfaffl  4 , Erika von Mutius  10 , Klaus F Rabe  1   2 , Gesine Hansen  11 , Matthias V Kopp  8   9 , Inke R König  12 , Sabine Bartel  3   13
Affiliations

RNA-seq-based profiling of extracellular vesicles in plasma reveals a potential role of miR-122-5p in asthma

Thomas Bahmer et al. Allergy. 2021 Jan.
No abstract available

Keywords: asthma; biomarkers; endotypes; microRNA; precision medicine.

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Conflict of interest statement

This study was supported by an unrestricted grant (Next Generation Award) from Bencard Allergie GmbH to SB, which did not influence the content of the manuscript or the conclusions drawn. The authors declare no further conflict of interest relating to this manuscript.

Figures

FIGURE 1
FIGURE 1
small RNA sequencing from plasma EVs. A, EV concentration (left panel), size distribution, and median size (right panels) in plasma EV isolations from healthy controls (HC, n = 16), mild‐to‐moderate (AM, n = 15), or severe asthma (AS, n = 14) assessed by nanoparticle tracking analysis (lines represent median). B, Protein content of qEV fractions in mg/mL, assessed by microBCA (n = 45 per fraction, mean ± SD). C, Sequencing distribution of small RNA classes in %, with relative distribution within mapped reads (right) (D and E) 10 top‐regulated miRNAs (sorted by log2 fold change) in asthma patients vs HC (D) and AM vs HC (left) and AS vs HC (right) (E); FDR = false discovery rate. F, Spearman's correlations of miR‐122‐5p (upper panels), miR‐3168 (middle panels), and miR‐191‐5p (lower panels) read counts with clinical characteristics of all included subjects (n = 45), and each graph depicts correlation co‐efficient R and P‐value
FIGURE 2
FIGURE 2
EV‐miRNA from plasma correlates with clinical characteristics of asthma. A and B, RT‐qPCR for miR‐122‐5p and miR‐191‐5p normalized to miR‐21 levels (reference) in (A) plasma EVs from healthy controls (HC, n = 26), all asthma (n = 51), mild‐to‐moderate (AM, n = 25) or severe asthma (AS, n = 26), or (B) sputum supernatant EVs from healthy controls (HC, n = 4) and patients with asthma (n = 10). All Mann–Whitney U to HC or AM. *P ≤ .05, **P ≤ .01, ***P ≤ .001. Graphs depict fold changes to healthy controls with lines indicating the median. (C) Ingenuity pathway analysis showing association of miR‐122‐5p and miR‐191‐5p with quantity of leukocytes, function of antigen‐presenting cells, activation of antigen‐presenting cells, function of Th2 cells, quantity of regulatory T lymphocytes, and differentiation of Th2 cells
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