QTL analysis of farinograph and mixograph related traits in spring wheat under heat stress conditions

Abstract

Farinograph and mixograph-related parameters are key elements in wheat end-products quality. Understanding the genetic control of these traits and the influence of environmental factors such as heat stress, and their interaction are critical for developing cultivars with improved for those traits. To identify QTL for six farinograph and three mixograph traits, two double haploid (DH) populations (Yecora Rojo × Ksu106 and Klasic × Ksu105) were used in experiments conducted at Riyadh and Al Qassim locations under heat stress. Single nucleotide polymorphism (SNP) markers were used to determine the number of QTLs controlling these parameters. The genetic analysis of farinograph and mixograph-related traits showed considerable variation with transgressive segregation regardless of heat stress conditions in both locations. A total of 108 additive QTLs were detected for the six farinograph and three mixograph traits in the Yecora Rojo × Ksu106 population in both locations under heat treatments. These QTLs were distributed over all 21 wheat chromosomes except 3A. Similarly, in Klassic × Ksu105 population, there were an additional 68 QTLs identified over the two locations and were allocated on all chromosomes except 1D, 2A, 6A, and 6D. In population (Yecora Rojo × Ksu106), the QTL on chromosome 7A (Excalibur_c62415_288) showed significant effects for farinograph and mixograph traits (FDDT, FDST, FBD, M × h8, and M × t) under normal and heat stress condition at both locations. Interestingly, several QTLs that are related to farinograph and mixograph traits, which showed stable expression under both locations, were detected on chromosome 7A in population (Klassic × Ksu105). Results from this study show the quantitative nature of the genetic control of the studied traits and constitute a step toward identifying major QTLs that can be sued molecular-marker assisted breeding to develop new improved quality wheat cultivars.

Keywords: Farinograph traits; Heat stress; Mixograph traits; QTL; SNP markers; Wheat quality.