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. 1988 Nov;85(21):8206-10.
doi: 10.1073/pnas.85.21.8206.

Stepwise intraclonal maturation of antibody affinity through somatic hypermutation

C Kocks  1 , K Rajewsky
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Stepwise intraclonal maturation of antibody affinity through somatic hypermutation

C Kocks et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

Using recombinant DNA techniques, we reconstructed a genealogical tree (Sablitzky, F., Wildner, G. & Rajewsky, K. (1985) EMBO J. 4, 345-350) that connects three clonally related B cells producing somatically mutated antibodies to a progenitor cell expressing a germ line-encoded antibody. The somatic mutants had been isolated from an in vivo immune response. The germ line-encoded progenitor antibody bound the antigen with high affinity. Intraclonal affinity maturation occurred stepwise over a 15-fold range.

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References

    1. EMBO J. 1983;2(8):1373-8 - PubMed
    1. EMBO J. 1988 Jul;7(7):1995-2001 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Q Rev Biophys. 1977 Feb;10(1):35-65 - PubMed
    1. Eur J Immunol. 1977 Oct;7(10):690 - PubMed

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