Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jul;10(7):324.
doi: 10.1007/s13205-020-02311-4. Epub 2020 Jun 29.

The involvement of the Type Six Secretion System (T6SS) in the virulence of Ralstonia solanacearum on brinjal

Trupti Asolkar  1   2 , Raman Ramesh  2
Affiliations

The involvement of the Type Six Secretion System (T6SS) in the virulence of Ralstonia solanacearum on brinjal

Trupti Asolkar et al. 3 Biotech. 2020 Jul.

Abstract

Ralstonia solanacearum is an important soil-borne plant pathogen which causes bacterial wilt in a large number of crops. Bacterial Type Six Secretion System (T6SS) is known to participate in pathogenesis, bacterial interaction and inter-bacterial competition. Contribution of T6SS in the virulence of R. solanacearum on eggplant (Solanum melongena L) is studied. In this study, five T6SS gene (ompA, vgrG3, hcp, tssH and tssM) mutants have been developed by insertional mutagenesis and the virulence of the mutants was evaluated on eggplant. In general, the T6SS mutants showed significant reduction of wilt on eggplant. R. solanacearum mutant of ompA gene significantly reduced the wilt from day five through day eight in petiole inoculation. In soil drench inoculation, R. solanacearum mutant of vgrG3 gene reduced the wilt on eggplant and was significantly different throughout the experimental period. Other mutants, viz., tssH, tssM and hcp, also reduced the wilt during the initial stages of disease development. This is the first report on the role of T6SS genes, ompA, vgrG3, hcp and tssH on virulence of R. solanacearum.

Keywords: Bacterial wilt; Eggplant; Mutants; Ralstonia solanacearum; T6SS; Virulence.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestOn behalf of all the authors, the corresponding author states that there is no conflict of interest.

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Asolkar T, Ramesh R. Identification of virulence factors and type III effectors of phylotype I, Indian Ralstonia solanacearum strains Rs-09-161 and Rs-10-244. J Genet. 2018;97:55–66. doi: 10.1007/s12041-018-0894-z. - DOI - PubMed
    1. Asolkar T, Ramesh R. Development of T3SS mutants (hrpB− and hrcV−) of Ralstonia solanacearum, evaluation of virulence attenuation in brinjal and tomato-A pre-requisite to validate T3Es of R. solanacearum. Indian J Microbiol. 2018;58:372–380. doi: 10.1007/s12088-018-0736-y. - DOI - PMC - PubMed
    1. Assefa M, Dawit W, Lencho A, Hunduma T. Assessment of wilt intensity and identification of causal fungal and bacterial pathogens on hot pepper (Capsicum annuum L.) in BakoTibbe and Nonno districts of west Shewa zone. Ethiopia Int J Phytopathol. 2015;4:21–28. doi: 10.33687/phytopath.004.01.0972. - DOI
    1. Badr S, Li Y, Duan K. Comparison of the structure, regulation and functions between type three and type six secretion system in gram-negative bacteria. J Med Microb Diagn. 2016;5:2161–2703. doi: 10.4172/2161-0703.1000243. - DOI
    1. Boyer F, Fichant G, Berthod J, Vandenbrouck Y, Attree I. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC genomics. 2009;10:104. doi: 10.1186/1471-2164-10-104. - DOI - PMC - PubMed

LinkOut - more resources