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. 2020 Jun 25;5(26):15812-15820.
doi: 10.1021/acsomega.0c00526. eCollection 2020 Jul 7.

Effect of a Nonionic Surfactant on Enzymatic Hydrolysis of Lignocellulose Based on Lignocellulosic Features and Enzyme Adsorption

Wen Wang  1 , Chaojun Wang  1   2 , Zahoor  1 , Xiaoyan Chen  1   2 , Qiang Yu  1 , Zhongming Wang  1 , Xinshu Zhuang  1 , Zhenhong Yuan  1   3
Affiliations

Effect of a Nonionic Surfactant on Enzymatic Hydrolysis of Lignocellulose Based on Lignocellulosic Features and Enzyme Adsorption

Wen Wang et al. ACS Omega. .

Abstract

Reduction in the adsorption of cellulase onto lignin has been thought to be the common reason for the improvement of enzymatic hydrolysis of lignocellulose (EHLC) by a nonionic surfactant (NIS). Few research studies have focused on the relationship between lignocellulosic features and NIS for improving EHLC. This study investigated the impact of Tween20 on the enzymatic hydrolysis and enzyme adsorption of acid-treated and alkali-treated sugarcane bagasse (SCB), cypress, and Pterocarpus soyauxii (PS) with and without being ground. After addition of Tween20, the adsorption of cellulase onto unground and ground alkali-treated SCB increased, and the unground acid-treated SCB exhibited little change in adsorption cellulase, while other unground and ground, treated samples showed decreased cellulase adsorption. Tween20 could improve the enzymatic hydrolysis of acid-treated SCB, while it had little influence on the enzymatic hydrolysis of other treated materials. After being ground, both cellulase adsorption and enzymatic hydrolysis of treated lignocelluloses increased, and Tween20 could enhance the enzymatic hydrolysis of acid-treated materials while hardly affected the enzymatic hydrolysis of alkali-treated materials. This indicated that the promotion effect of Tween20 on enzymatic hydrolysis of treated lignocellulose could not be mainly ascribed to the hindrance of Tween20 to cellulase adsorption on lignin but was related to the lignocellulosic features such as hemicellulose removal and surface morphology changes.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
FTIR spectra of raw and treated lignocelluloses in the range of 800–1900 cm–1: (a) raw samples, (b) acid-treated samples, and (c) alkali-treated samples.
Figure 2
Figure 2
Effect of Tween20 on the enzymatic hydrolysis of acid-treated and alkali-treated lignocelluloses with and without being ground: (a) glucan conversion of acid-treated lignocelluloses, (b) xylan conversion of acid-treated lignocelluloses, (c) glucan conversion of alkali-treated lignocelluloses, and (d) xylan conversion of alkali-treated lignocelluloses.
Figure 3
Figure 3
Effect of Tween20 on the adsorption of enzyme protein onto acid-treated (a) and alkali-treated (b) lignocelluloses with and without being ground.
Figure 4
Figure 4
SEM images of raw and treated lignocelluloses.
Figure 5
Figure 5
Crystallinity index of raw and treated lignocelluloses.
See this image and copyright information in PMC

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