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. 2020 Jul 10;12(7):1531.
doi: 10.3390/polym12071531.

Novel Exopolysaccharide Produced from Fermented Bamboo Shoot-Isolated Lactobacillus Fermentum

Thi Bich Thuy Do  1 , Thi Ai Luyen Tran  2 , Thi Van Thi Tran  3 , Trung Hieu Le  3 , Vijay Jayasena  4 , Thi Hong Chuong Nguyen  5   6 , Chinh Chien Nguyen  5   6 , Soo Young Kim  7 , Quyet Van Le  5   7
Affiliations

Novel Exopolysaccharide Produced from Fermented Bamboo Shoot-Isolated Lactobacillus Fermentum

Thi Bich Thuy Do et al. Polymers (Basel). .

Abstract

This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in the BCCM/LMG Bacteria Collection, Ghent University, Belgium by the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 104 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.

Keywords: Lactobacillus fermentum; exopolysaccharide; gas–liquid chromatography–mass spectrometry; lactic acid bacteria; nuclear magnetic resonance spectroscopy; phenylalanyl-tRNA synthase gene.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The production of exopolysaccharide (EPS) from some bacterial strains of fermented bamboo shoots and fermented meat. Data are means SD of EPS production from triplicate experiments. Bars with different letters are significantly different at p < 0.05.
Figure 2
Figure 2
The molecular mass chromatogram of EPS from Lb. fermentum MC3 obtained by gel permeation HPLC MC3.
Figure 3
Figure 3
1H NMR spectrum of EPS from Lb. fermentum MC3.
Figure 4
Figure 4
13C NMR spectrum of EPS from Lb. fermentum MC3.
Figure 5
Figure 5
2D 1H–13C heteronuclear single-quantum correlation spectroscopy (HSQC) spectrum of EPS from Lb. fermentum MC3; a (overall); b (expansion 1); and, c (expansion 2).
Figure 6
Figure 6
2D 1H–1H correlation spectroscopy (COSY) spectrum of EPS from Lb. fermentum MC3.
Figure 7
Figure 7
Heteronuclear multiple-bond correlation spectroscopy (HMBC) spectrum of EPS from Lb. fermentum MC3.
Figure 8
Figure 8
Nuclear Overhauser effect spectroscopy (NOESY) spectrum of EPS from Lb. fermentum MC3.
Figure 9
Figure 9
Outline of the biosynthesis of hetero EPS by lactic acid bacteria (LAB).
See this image and copyright information in PMC

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