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Comparative Study
. 2020 Sep 22;58(10):e01622-20.
doi: 10.1128/JCM.01622-20. Print 2020 Sep 22.

Comparison of Extraction Methods and Thermocyclers for SARS-CoV-2 Molecular Detection Using Clinical Specimens

Eric M Ransom  1 , Robert F Potter  1 , Meghan A Wallace  1 , Kaitlin F Mitchell  1 , Melanie L Yarbrough  1 , C A Burnham  1   2   3   4 , Neil W Anderson  5 , Bijal A Parikh  5
Affiliations
Comparative Study

Comparison of Extraction Methods and Thermocyclers for SARS-CoV-2 Molecular Detection Using Clinical Specimens

Eric M Ransom et al. J Clin Microbiol. .
No abstract available

Keywords: ABI 7500; COVID-19; EZ1; KingFisher; Lyra; Lyra SARS-CoV-2; Rotor-Gene Q; coronavirus; easyMAG.

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Figures

FIG 1
FIG 1
Comparison of extraction methods using 10 clinical nasopharyngeal swab specimens. A single sample that was not detected is shown at 30 CT (the internal processing control [PRC] from the Lyra kit was detected in this sample). Statistical analyses performed in GraphPad Prism v8.0. Note that the Lyra assay removes the first 10 CT cycles (add +10 CT values to compare with conventional methods).
FIG 2
FIG 2
Precision and efficiency analyses. Technical replicates of a clinical sample: neat, 1:10, and 1:100. Samples were amplified on the 7500 Fast system. A single sample that was not detected is included at 30 CT (the internal processing control [PRC] from the Lyra kit was detected in this sample). Note that the Lyra assay removes the first 10 CT cycles (add +10 CT values to compare with conventional methods).
See this image and copyright information in PMC

References

    1. Xiao AT, Tong YX, Gao C, Zhu L, Zhang YJ, Zhang S. 2020. Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: a descriptive study. J Clin Virol 127:104346. doi:10.1016/j.jcv.2020.104346. - DOI - PMC - PubMed
    1. Roser M, Ritchie H, Ortiz-Ospina E, Hasell J. 2020. Coronavirus pandemic (COVID-19). Our World in Data. https://ourworldindata.org/coronavirus. Accessed 2 June 2020.
    1. Pabbaraju K, Wong AA, Douesnard M, Ma R, Gill K, Dieu P, Fonseca K, Zelyas N, Tipples GA. 2020. A public health laboratory response to the COVID-19 pandemic. J Clin Microbiol doi:10.1128/JCM.01110-20. - DOI - PMC - PubMed
    1. Mitchell SL, St. George K, Rhoads DD, Butler-Wu SM, Dharmarha V, McNult P, Miller MB. 2020. Understanding, verifying and implementing Emergency Use Authorization molecular diagnostics for the detection of SARS-CoV-2 RNA. J Clin Microbiol doi:10.1128/JCM.00796-20. - DOI - PMC - PubMed
    1. Fisher T. 2016. KingFisher Flex user manual. https://assets.thermofisher.com/TFS-Assets/BID/manuals/KingFisher%20Flex.... Accessed 2 June 2020.

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