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. 2020 Jun 29:7:100979.
doi: 10.1016/j.mex.2020.100979. eCollection 2020.

Percutaneous cannulation for extracorporeal membrane oxygenation (ECMO): A method for pig experimental models

Caroline Fritz  1 , Nicolas Viault  1 , Baptiste Fohlen  1 , Maximilian Edlinger-Stanger  2 , Hanna McGregor  3 , Bernard Cholley  1 , Neal Fleming  4
Affiliations

Percutaneous cannulation for extracorporeal membrane oxygenation (ECMO): A method for pig experimental models

Caroline Fritz et al. MethodsX. .

Abstract

Pigs are often used for experimental models of cardiogenic shock, cardiac arrest or acute lung injury with veno-arterial (VA) or veno-venous (VV) extracorporeal membrane oxygenation (ECMO) implementation. Percutaneous (as opposed to surgical) cannulation in experimental models has potential advantages, including, less surgical trauma or stressful stimuli and less bleeding complications when compared to open chest cannulation. However, pig anatomy can also be a challenge because of the deep location and angled anatomy of the femoral artery. The Seldinger technique and the use of a percutaneous cannulation kit is feasible in pigs. Summarized here we present (Graphical Abstract):•Percutaneous ECMO cannulation method for non-cardiac surgeon.•Establishment of this simple and rapid methods for pig experimental models.•Predictable complications of this method.

Keywords: Experimental pig model; Extracorporeal membrane oxygenation; Percutaneous cannulation; Ultrasound-guided cannulation.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image, graphical abstract
Graphical abstract
Fig 2
Fig. 2
Procedure for cannulae connection to ECMO circuit and pump start. A: cannulae in place. B. Connection of the cannula to the ECMO circuit while dripping saline over the junction to avoid air bubbles in the circuit. C. Cannulae and circuit are connected. D. Starting the centrifugal pump prior to remove the venous clamp. E. Remove the arterial line clamp after the venous clamp. F. Ensure that the two cannulae have different color.
Fig 1
Fig. 1
Femoral percutaneous cannulation. A: ultrasound allows localization of the femoral artery and vein. B: Needle puncture under ultrasound guidance. C: arterial and venous guidewires in place. D: percutaneous insertion kit ready with two long wires. E: Arterial dilatation, venous canula already in place. F: arterial and venous cannulae in place and clamped before ECMO circuit connection.
See this image and copyright information in PMC

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