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. 2020 Sep;31(9):1944-1947.
doi: 10.1681/ASN.2020050683. Epub 2020 Jul 16.

Histopathologic and Ultrastructural Findings in Postmortem Kidney Biopsy Material in 12 Patients with AKI and COVID-19

Pouneh Golmai  1 , Christopher P Larsen  2 , Maria V DeVita  1 , Samuel J Wahl  3 , Astrid Weins  4 , Helmut G Rennke  4 , Vanesa Bijol  5 , Jordan L Rosenstock  6
Affiliations

Histopathologic and Ultrastructural Findings in Postmortem Kidney Biopsy Material in 12 Patients with AKI and COVID-19

Pouneh Golmai et al. J Am Soc Nephrol. 2020 Sep.
No abstract available

Keywords: Acute kidney injury; COVID-19; SARS-CoV-2; biopsy; pathology; severe acute respiratory syndrome coronavirus 2; virion.

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Figures

Figure 1.
Figure 1.
Electron microscopy and in situ hybridization for SARS-CoV-2 reveal no evidence of viral presence in kidney biopsy material. (A) Electron micrograph of a proximal tubule epithelial cell with many clathrin-coated vesicles aggregated in cytosol, closely resembling viral particles. Original magnification, ×10,000. The white square in (A) is shown magnified in (B) and (C). Original magnification, ×50,000 in B; ×100,000 in C. (D) Multivesicular bodies in the podocyte cytoplasm. Original magnification, ×50,000. (E) RNAScope using probes directed against SARS-CoV-2 shows absence of signal in the patient’s kidney parenchyma. Original magnification, ×200. (F) Adequate quality of tissue is confirmed by RNAScope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B using 3,3′-diaminobenzidine (brown) chromogen. Original magnification, ×200. (G) Negative control of the in situ hybridization assay. Original magnification, ×200.
See this image and copyright information in PMC

Comment in

  • Authors' Reply.
    Rosenstock JL, Bijol V. Rosenstock JL, et al. J Am Soc Nephrol. 2021 Jan;32(1):255-256. doi: 10.1681/ASN.2020101479. Epub 2020 Nov 5. J Am Soc Nephrol. 2021. PMID: 33155987 Free PMC article. No abstract available.
  • Post-Mortem Diagnostics in COVID-19 AKI, More Often but Timely.
    Zijlstra JG, van Meurs M, Moser J. Zijlstra JG, et al. J Am Soc Nephrol. 2021 Jan;32(1):255. doi: 10.1681/ASN.2020091263. Epub 2020 Nov 5. J Am Soc Nephrol. 2021. PMID: 33170136 Free PMC article. No abstract available.

References

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