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. 1977 Jun;73(3):783-8.
doi: 10.1083/jcb.73.3.783.

Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy

M H Heggeness, J F Ash

Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy

M H Heggeness et al. J Cell Biol. 1977 Jun.

Abstract

A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.

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