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. 2020 Sep-Oct;25(5):714-719.
doi: 10.1016/j.rpor.2020.06.007. Epub 2020 Jul 10.

Apoptosis related microRNAs and MGMT in glioblastoma cell lines submitted to treatments with ionizing radiation and temozolomide

Felipe Amstalden Trevisan  1 , Andressa Romualdo Rodrigues  2 , Fermino Sanches Lizarte Neto  1 , Fernanda Maris Peria  3 , Múcio Luiz de Assis Cirino  1 , Daniela Pretti da Cunha Tirapelli  1 , Carlos Gilberto Carlotti Júnior  1
Affiliations

Apoptosis related microRNAs and MGMT in glioblastoma cell lines submitted to treatments with ionizing radiation and temozolomide

Felipe Amstalden Trevisan et al. Rep Pract Oncol Radiother. 2020 Sep-Oct.

Abstract

Aim: To evaluate the effect of radiotherapy and temozolomide on the expression of miRNAs apoptotic (miRNAs-21, -221, -222 (anti-apoptotic) and miRNAs-15a, -16 (pro-apoptotic)) and the gene MGMT in glioblastoma cell lines.

Background: The limited knowledge of the molecular biology of malignant gliomas may hinder the development of therapeutic modalities. In this scenario, one of the greatest advances of recent years was the identification of microRNAs. These molecules have an important role in biological processes involving cancer, including glioblastoma.

Materials and methods: Trypan blue was used to verify the cell viability, and real time PCR to quantify the expression of microRNAs and gene 24, 48 and 120 h after exposure to treatments.

Results: There was a statistically significant decrease of expression of miR-15a between 48 and 120 h in line T98 G treated with radiation, increased expression of miR-15a between 24 and 120 h in line U251 treated with radiation and temozolomide, and increased expression of miR-16 between 24 and 120 h in line U251 treated with radiation alone and when combined with temozolomide. There was a decrease in MGMT gene expression, between 24 and 48 h in U343 cells treated with temozolomide.

Conclusions: Ionizing radiation and temozolomide modified the expression of miRNAs studied and MGMT.

Keywords: Apoptosis; Glioblastoma; Ionizing radiation; microRNAs.

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Figures

Fig. 1
Fig. 1
Cell viability versus treatment modality for T98 G, U251 and U343 glioblastoma cell lines.
Fig. 2
Fig. 2
Expression of the MGMT gene, for each time, according to type of therapy applied for the T-98 G glioblastoma cell line.
Fig. 3
Fig. 3
Expression of the MGMT gene, for each time, according to type of therapy applied for the U251 glioblastoma cell line.
Fig. 4
Fig. 4
Expression of the MGMT gene, for each time, according to type of therapy applied for the U343 glioblastoma cell line.
Fig. 5
Fig. 5
MicroRNAs expression in T98 G glioblastoma cell line analyzed 24, 48 and 120 h (a, b and c, respectively) after treatment.
Fig. 6
Fig. 6
MicroRNAs expression in U251 glioblastoma cell line analyzed 24, 48 and 120 h (a, b and c, respectively) after treatment.
Fig. 7
Fig. 7
MicroRNAs expression in U343 glioblastoma cell line analyzed 24, 48 and 120 h (a, b and c, respectively) after treatment.
See this image and copyright information in PMC

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