Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jul 13;6(2):00324-2020.
doi: 10.1183/23120541.00324-2020. eCollection 2020 Apr.

False-negative RT-PCR in SARS-CoV-2 disease: experience from an Italian COVID-19 unit

Marcello Di Paolo  1 , Alessandra Iacovelli  1 , Federica Olmati  1 , Ilaria Menichini  1 , Alessandra Oliva  1 , Martina Carnevalini  1 , Elda Graziani  1 , Claudio Maria Mastroianni  1 , Paolo Palange  1
Affiliations

False-negative RT-PCR in SARS-CoV-2 disease: experience from an Italian COVID-19 unit

Marcello Di Paolo et al. ERJ Open Res. .

Abstract

False-negative cases of #COVID19 are being increasingly reported. Laboratory diagnosis through RT-PCR testing alone lacks adequate sensitivity to be recommended as the only valid criterion to confirm COVID-19 diagnosis. https://bit.ly/2BLFnEe.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: M. Di Paolo has nothing to disclose. Conflict of interest: A. Iacovelli has nothing to disclose. Conflict of interest: F. Olmati has nothing to disclose. Conflict of interest: I. Menichini has nothing to disclose. Conflict of interest: A. Oliva has nothing to disclose. Conflict of interest: M. Carnevalini has nothing to disclose. Conflict of interest: E. Graziani has nothing to disclose. Conflict of interest: C.M. Mastroianni has nothing to disclose. Conflict of interest: P. Palange has nothing to disclose.

References

    1. World Health Organization Coronavirus disease 2019 (COVID-19) Situation Report – 126 www.who.int/docs/default-source/coronaviruse/situation-reports/20200525-.... Date last updated: 25th May 2020. Date last accessed: 26th May 2020.
    1. World Health Organization Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases. Interim guidance. WHO REFERENCE NUMBER: WHO/COVID-19/laboratory/2020.5 www.who.int/publications-detail/laboratory-testing-for-2019-novel-corona.... Date last updated: 19th March 2020. Date last accessed: 15th May 2020.
    1. European Centre for Disease Prevention and Control Laboratory support for COVID-19 in the EU/EEA www.ecdc.europa.eu/en/novel-coronavirus/laboratory-support. Date last updated: 15th April 2020. Date last accessed: 15th May 2020.
    1. Centers for Disease Control and Prevention Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens from Persons for Coronavirus Disease 2019 (COVID-19) www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html. Date last updated: 5th May 2020. Date last accessed: 15th May 2020.
    1. Huang P, Liu T, Huang L, et al. . Use of chest CT in combination with negative RT-PCR assay for the 2019 novel coronavirus but high clinical suspicion. Radiology 2020; 295: 22–23. doi:10.1148/radiol.2020200330 - DOI - PMC - PubMed

LinkOut - more resources