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. 2020 Jul 20;20(1):213.
doi: 10.1186/s12866-020-01881-w.

High prevalence of t895 and t9364 spa types of methicillin-resistant Staphylococcus aureus in a tertiary-care hospital in Mexico: different lineages of clonal complex 5

C Negrete-González  1 , E Turrubiartes-Martínez  1   2 , O G Galicia-Cruz  3 , D E Noyola  4 , G Martínez-Aguilar  5 , L F Pérez-González  6 , R González-Amaro  7 , P Niño-Moreno  8   9
Affiliations

High prevalence of t895 and t9364 spa types of methicillin-resistant Staphylococcus aureus in a tertiary-care hospital in Mexico: different lineages of clonal complex 5

C Negrete-González et al. BMC Microbiol. .

Abstract

Background: Staphylococcus aureus is a leading cause of broad-spectrum infections both in the community and within healthcare settings. Methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health issue. The aim of this study was to examine the clinical and molecular characteristics of Staphylococcus aureus isolates and to define the population structure and distribution of major MRSA clones isolated in a tertiary-care hospital in Mexico.

Results: From April 2017 to April 2018, 191 Staphylococcus aureus isolates were collected. The frequency of MRSA was 26.7%; these strains exhibited resistance to clindamycin (84.3%), erythromycin (86.2%), levofloxacin (80.3%), and ciprofloxacin (86.3%). The majority of MRSA strains harbored the SCCmec type II (76.4%) and t895 (56.8%) and t9364 (11.7%) were the most common spa types in both hospital-associated MRSA and community-associated MRSA isolates. ST5-MRSA-II-t895 (New York /Japan clone) and ST1011-MRSA-II-t9364 (New York /Japan-Mexican Variant clone) were the most frequently identified clones. Furthermore, different lineages of Clonal Complexes 5 (85.4%) and 8 (8.3%) were predominantly identified in this study.

Conclusion: Our study provides valuable information about the epidemiology of MRSA in a city of the central region of Mexico, and this is the first report on the association between t895 and t9364 spa types and ST5 and ST1011 lineages, respectively. These findings support the importance of permanent surveillance of MRSA aimed to detect the evolutionary changes of the endemic clones and the emergence of new strains.

Keywords: Clonal complex 5-ST1011; Methicillin-resistant Staphylococcus aureus; New York/Japan-Mexican variant clone; SCCmec type II; Spa type t895; Spa type t9364; Spa-typing.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Number of cases of S. aureus infection in each epidemiological week. Black bars correspond to MRSA isolates and grey bars to MSSA strains
Fig. 2
Fig. 2
Dendrogram of MRSA strains. Dendrogram constructed using the unweighted pair group method with arithmetic average (UPGMA) based on pairwise similarity values of spa types from 48 characterized MRSA strains. The scale corresponds to the percent of similarity. Blue branch corresponds to cluster 1, red branch to cluster 2, green branch to cluster 3, and orange branch to cluster 4
See this image and copyright information in PMC

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