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. 2020 Jun 23:2020:8888615.
doi: 10.1155/2020/8888615. eCollection 2020.

Response and Adaptation of Microbial Community in a CANON Reactor Exposed to an Extreme Alkaline Shock

Ruili Yang  1   2 , Wenlong Mao  1   3 , Xiaojun Wang  1 , Zhaoji Zhang  1 , Junbin Wu  1 , Shaohua Chen  1
Affiliations

Response and Adaptation of Microbial Community in a CANON Reactor Exposed to an Extreme Alkaline Shock

Ruili Yang et al. Archaea. .

Abstract

Responses of a microbial community in the completely autotrophic nitrogen removal over nitrite (CANON) process, which was shocked by a pH of 11.0 for 12 h, were investigated. During the recovery phase, the performance, anaerobic ammonia oxidation (anammox) activity, microbial community, and correlation of bacteria as well as the influencing factors were evaluated synchronously. The performance of the CANON process deteriorated rapidly with a nitrogen removal rate (NRR) of 0.13 kg·m-3·d-1, and Firmicutes, spore-forming bacteria, were the dominant phyla after alkaline shock. However, it could self-restore within 107 days after undergoing four stages, at which Planctomycetes became dominant with a relative abundance of 64.62%. Network analysis showed that anammox bacteria (Candidatus Jettenia, Kuenenia, and Brocadia) were positively related to some functional bacteria such as Nitrosomonas, SM1A02, and Calorithrix. Canonical correspondence analysis presented a strong correlation between the microbial community and influencing factors during the recovery phase. With the increase of nitrogen loading rate, the decrease of free nitrous acid and the synergistic effects, heme c content, specific anammox activity (SAA), NRR, and the abundance of dominant genus increased correspondingly. The increase of heme c content regulates the quorum sensing system, promotes the secretion of extracellular polymeric substances, and further improves SAA, NRR, and the relative abundance of the dominant genus. This study highlights some implications for the recovery of the CANON reactor after being exposed to an alkaline shock.

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Conflict of interest statement

The authors declare that there is no conflict of interest regarding the publication of this article.

Figures

Figure 1
Figure 1
Schematic of the experimental device.
Figure 2
Figure 2
Shifts in nitrogen concentration (a), nitrogen removal efficiency (b), nitrogen loading rate and removal rate (c), stoichiometric ratio (d), and free ammonia and free nitrous acid (e) of the CANON process after alkaline shock.
Figure 3
Figure 3
Variations of SAA (a), heme c (b), EPS (c), and signalling molecule (d) content in the CANON process after alkaline shock.
Figure 4
Figure 4
Bacterial community composition at the phylum (a) and genus (b) levels in the CANON process after alkaline shock.
Figure 5
Figure 5
(a) Cooccurring network analysis of dominant microflora, (b) CCA of microbial communities and the influencing factors, and (c) correlation among dominant microflora, in the CANON process exposed to alkaline shock. The cooccurring network is coloured by the modularity class. Each node represents a dominant genus. Red and green lines represent positive and negative correlations, respectively. Node sizes are weighted by the node degree (numbers of connections).
See this image and copyright information in PMC

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