Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Sep;34(9):e23371.
doi: 10.1002/jcla.23371. Epub 2020 Jul 22.

Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Najeeb Ullah Khan  1 , Lubna Danish  2 , Hydayat Ullah Khan  3 , Maryam Shah  1 , Muhammad Ismail  4 , Ijaz Ali  5 , Arnolfo Petruzziello  6 , Rocco Sabatino  7 , Annunziata Guzzo  8 , Gerardo Botti  9 , Aqib Iqbal  1
Affiliations

Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Najeeb Ullah Khan et al. J Clin Lab Anal. 2020 Sep.

Abstract

Background: Dengue is a viral disease, transmitted by infected Aedes aegypti and Aedes albopictus female mosquitoes. Worldwide, 96 million infections were estimated in 2010. The dengue virus comprises four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) which belong to the genus Flavivirus. Determining the serotypes during dengue outbreaks is crucial for its effective management in terms of diagnostics improvement and polyvalent vaccine development. The aim of the present study is to determine the prevalence rate of dengue virus serotypes in the samples collected from patients during the 2017 outbreak in Khyber Pakhtunkhwa, Pakistan.

Methods: A total of 800 ELISA-positive samples were collected, of which 513 (290 males, 223 females) samples were confirmed positive by PCR.

Results: Out of 513, 25 were found serotype 1 (5%), 196 were serotype 2 (38%), 192 were serotype 3 (37%), 56 were serotype 4 (11%), and 44 (8%) were found to have mix serotypes.

Conclusion: We can conclude that serotypes 2 and 3 of dengue virus were the predominated serotypes of dengue virus in the 2017 outbreak in Peshawar, capital city of Khyber Pakhtunkhwa, Pakistan.

Keywords: KP-Pakistan; dengue; dengue virus; prevalence; serotyping.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there is no conflict of interests regarding the publication of this paper.

Figures

Figure 1
Figure 1
Active dengue virus infection. A, 800 samples were IgM/IgG positive, which was confirmed by ELISA (filled bar). All ELISA‐positive samples were further confirmed by RT‐PCR for active DENV infection (empty bar). B, Shows active infection in male and female
Figure 2
Figure 2
Age‐wise distribution of DENV‐infected patients. Empty bars represent the number of male patients belonging to different age groups, while bars with patterns show the female patients in three age groups
Figure 3
Figure 3
Serotype distribution of DENV in Peshawar, KPK. A, All DENV‐positive samples were further analyzed to identify the specific serotype. Bar graph represents the serotype distribution in male and female patients. B, Pie chart shows the percentage of all serotypes in DENV‐positive samples
See this image and copyright information in PMC

References

    1. Bhatt S, Gething PW, Brady OJ, et al. The global distribution and burden of dengue. Nature. 2013;496:504‐507. - PMC - PubMed
    1. WHO/ TDR . Dengue: guidelines for diagnosis, treatment, prevention, and control. Special Programme for Research and Training in Tropical Diseases. 2009. 147.
    1. Rasheed SB, Butlin RK, Boots M. A review of dengue as an emerging disease in Pakistan. Public Health. 2013;127:11‐17. - PubMed
    1. Humayoun MA, Waseem T, Jawa AA, Hashmi MS, Akram J. Multiple dengue serotypes and high frequency of dengue hemorrhagic fever at two tertiary care hospitals in Lahore during the 2008 dengue virus outbreak in Punjab, Pakistan. Int J Infect Dis. 2010;14:e54‐e59. - PubMed
    1. Ooi EE, Gubler DJ. Dengue in Southeast Asia: epidemiological characteristics and strategic challenges in disease prevention. Cad Saude Publica. 2009;25(Suppl 1):115‐124. - PubMed