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. 2020 Jul 23;20(1):349.
doi: 10.1186/s12870-020-02553-7.

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers

Tao Jiang  1 , Meidi Zhang  2 , Chunxiu Wen  1 , Xiaoliang Xie  1 , Wei Tian  1 , Saiqun Wen  1 , Ruike Lu  1 , Lingdi Liu  3
Affiliations

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers

Tao Jiang et al. BMC Plant Biol. .

Abstract

Background: The objectives of this study were to reveal the anthocyanin biosynthesis metabolic pathway in white and purple flowers of Salvia miltiorrhiza using metabolomics and transcriptomics, to identify different anthocyanin metabolites, and to analyze the differentially expressed genes involved in anthocyanin biosynthesis.

Results: We analyzed the metabolomics and transcriptomics data of S. miltiorrhiza flowers. A total of 1994 differentially expressed genes and 84 flavonoid metabolites were identified between the white and purple flowers of S. miltiorrhiza. Integrated analysis of transcriptomics and metabolomics showed that cyanidin 3,5-O-diglucoside, malvidin 3,5-diglucoside, and cyanidin 3-O-galactoside were mainly responsible for the purple flower color of S. miltiorrhiza. A total of 100 unigenes encoding 10 enzymes were identified as candidate genes involved in anthocyanin biosynthesis in S. miltiorrhiza flowers. Low expression of the ANS gene decreased the anthocyanin content but enhanced the accumulation of flavonoids in S. miltiorrhiza flowers.

Conclusions: Our results provide valuable information on the anthocyanin metabolites and the candidate genes involved in the anthocyanin biosynthesis pathways in S. miltiorrhiza.

Keywords: Anthocyanin; Medicinal plant; Metabolite; S. Miltiorrhiza; Transcriptome.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Measurement of total flavonoids and anthocyanins in flowers of S. miltiorrhiza
Fig. 2
Fig. 2
Flavonoid metabolites in PFSM and WFSM
Fig. 3
Fig. 3
Relative contents of flavonoid and anthocyanin metabolites in PFSM and WFSM
Fig. 4
Fig. 4
Transcriptome analysis of genes in PFSM and WFSM
Fig. 5
Fig. 5
Analysis of genes in PFSM and WFSM
Fig. 6
Fig. 6
Biosynthetic pathway of anthocyanin in PFSM and WFSM
Fig. 7
Fig. 7
Sequence alignment and phylogenetic tree of SmANS protein (SMil_00006040)
Fig. 8
Fig. 8
Relative Expression levels of 12 genes involved in the anthocyanin biosynthetic pathway by qRT-PCR analysis
See this image and copyright information in PMC

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