Relationship between inflammatory biomarkers and oxidative stress with uterine health in dairy cows with different dry period lengths

Abstract

Earlier studies indicated that the inflammatory status of dairy cows in early lactation could not be fully explained by the negative energy balance (NEB) at that moment. The objective of the present study was to determine relationships between inflammatory biomarkers and oxidative stress with uterine health in dairy cows after different dry period lengths. Holstein-Friesian dairy cows were assigned to one of three dry period lengths (0-, 30-, or 60-d) and one of two early lactation rations (glucogenic or lipogenic ration). Cows were fed either a glucogenic or lipogenic ration from 10-d before the expected calving date. Part of the cows which were planned for a 0-d dry period dried themselves off and were attributed to a new group (0 → 30-d dry period), which resulted in total in four dry period groups. Blood was collected (N = 110 cows) in weeks -3, -2, -1, 1, 2, and 4 relative to calving to determine biomarkers for inflammation, liver function, and oxidative stress. Uterine health status (UHS) was monitored by scoring vaginal discharge (VD) based on a 4-point scoring system (0, 1, 2, or 3) in weeks 2 and 3 after calving. Cows were classified as having a healthy uterine environment (HU, VD score = 0 or 1 in both weeks 2 and 3), nonrecovering uterine environment (NRU, VD score = 2 or 3 in week 3), or a recovering uterine environment (RU, VD score = 2 or 3 in week 2 and VD score= 0 or 1 in week 3). Independent of dry period length, cows with NRU had higher plasma haptoglobin (P = 0.05) and lower paraoxonase levels (P < 0.01) in the first 4 weeks after calving and lower liver functionality index (P < 0.01) compared with cows with HU. Cows with NRU had lower plasma albumin (P = 0.02) and creatinine (P = 0.02) compared with cows with a RU, but not compared with cows with HU. Independent of UHS, cows with a 0 → 30-d dry period had higher bilirubin levels compared with cows with 0-, 30-, or 60-d dry period (P < 0.01). Cows with RU and fed a lipogenic ration had higher levels of albumin in plasma compared with cows with NRU and fed a lipogenic ration (P < 0.01). In conclusion, uterine health was related to biomarkers for inflammation (haptoglobin and albumin) and paraoxonase in dairy cows in early lactation. Cows which were planned for a 0-d dry period, but dried themselves off (0 → 30-d dry period group) had higher bilirubin levels, which was possibly related to a more severe NEB in these cows. Inflammatory biomarkers in dairy cows in early lactation were related to uterine health in this period.

Keywords: cattle; continuous milking; inflammation; oxidative stress; uterine health.

Figure 1.

(A) Albumin concentration in plasma for cows with different UHS (HU, RU, or NRU) and fed either a glucogenic or lipogenic ration. (B) Albumin concentration in plasma of cows with different dry period lengths (0-, 30-, 0 → 30-, or 60-d dry period) and different parities (3, 4, or >4). Values represent means ± SEM. Bar with different superscripts within dry period length (D), ration (R), or parity (P) class differ (P < 0.05).

Figure 2.

Inflammatory biomarkers, oxidative stress (ROM, and FRAP) and creatinine in plasma per week per class of UHS; HU; VD score: 0 or 1 in week 2 and score 0 or 1 in week 3 (n = 17), a RU; VD score: 2 or 3 in week 2 and score 0 or 1 in week 3 (n = 31), and a NRU; VD score: 2 or 3 in week 3 (n = 43) (Mean ± SEM). Significant UHS group differences within week are indicated by asterisk (P < 0.05).

Figure 2.

Inflammatory biomarkers, oxidative stress (ROM, and FRAP) and creatinine in plasma per week per class of UHS; HU; VD score: 0 or 1 in week 2 and score 0 or 1 in week 3 (n = 17), a RU; VD score: 2 or 3 in week 2 and score 0 or 1 in week 3 (n = 31), and a NRU; VD score: 2 or 3 in week 3 (n = 43) (Mean ± SEM). Significant UHS group differences within week are indicated by asterisk (P < 0.05).