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. 2020 Aug;34(8):e23296.
doi: 10.1002/jcla.23296. Epub 2020 Jul 24.

Ion chromatography as candidate reference method for the determination of chloride in human serum

Min Shen  1   2 , Minmin Tu  1 , Wei Zhang  1 , Jihua Zou  1 , Man Zhang  2 , Zheng Cao  3 , Bingde Zou  1
Affiliations

Ion chromatography as candidate reference method for the determination of chloride in human serum

Min Shen et al. J Clin Lab Anal. 2020 Aug.

Abstract

Background: We developed an ion chromatography (IC) method for measurement of chloride in human serum which was regarded as a simple, rapid, accurate, and sensitive technique. The method will be hopefully selected as a candidate reference method.

Method: Serum aliquots of 0.1 mL were diluted 500 times with Milli-Q water, and chloride in serum samples was measured by IC with a gradient elution procedure using a KOH eluent generator.

Results: Based on the data, chloride in human serum was well detected by IC. The calibration curve for chloride was linear in the concentration range from 0 to 0.42 mmol/L with a correlation coefficient of .99995 under the optimum experimental conditions. The chloride concentration had a good linear relationship with the peak areas of chloride. This method was sensitive because of the low limit of detection (LOD) and the low limit of quantification (LOQ) 9.87 × 10-5 mmol/L and 3.27 × 10-4 mmol/L, respectively. Besides, the method was highly precise with the within-run coefficient of variations (CVs) for the measurement of low, medium, and high concentration level samples 0.32%, 0.73%, and 0.50%. As for the evaluation of accuracy, the biases were less than ±1% and 2% by comparing with National Institute of Science and Technology (NIST) standard material SRM 956d and 2013-2018 IFCC-RELA samples, respectively. Finally, the biases between IC method and the inductively coupled plasma mass spectrometry (ICP-MS) method were less than 1% which showed good agreement.

Conclusion: Ion chromatography is a simple sample treatment procedure for the determination of chloride in human serum with high sensitivity and specificity. The proposed method could be recommended as a candidate reference method for the determination of serum chloride in human serum.

Keywords: candidate reference method; ion chromatography; serum chloride.

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Figures

Figure 1
Figure 1
Serum chromatogram after dilution
Figure 2
Figure 2
The standard curve of chloride
Figure 3
Figure 3
The comparison of ICP‐MS and IC
See this image and copyright information in PMC

References

    1. Rayana MCB, Burnett RW, Covington AK, et al. Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division, Committee on Point of Care Testing and Working Group on Selective Electrodes. Clin Chem Lab Med. 2006;44(3):346‐352. - PubMed
    1. Lingane JJ. Automatic coulometric titration with eleectrolytically denerated silver ion:determination of chliride, bromide and lodide ions. Anal chem. 1954;26(4):622‐626.
    1. Velapoldi RA, Paule RC, Schaffer R, et al. Standard Reference materials: Areference method for the determination of chloride in serum. Washington, D.C: U.S Government Printing Office; 1979.
    1. Lyon TDB, Fell GS, Hutton RC, et al. Elimination of chloride interence on the determination of selenium in serum by inductively coupled plasma mass spectrometry. J Anal At Spectrom. 1988;3:601‐603.
    1. Collie JT, Massie RJ, Jones OAH, et al. A candidate reference method using ICP‐MS for sweat chloride quantification. Clin Chem Lab Med. 2015;54(4):561‐567. - PubMed

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