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. 1977 Jun;22(3):619-25.
doi: 10.1128/JVI.22.3.619-625.1977.

In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174

R Mukai, M Hayashi

In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174

R Mukai et al. J Virol. 1977 Jun.

Abstract

An extract prepared from Escherichia coli cells infected with phi chi 174 bacteriophage was capable of incorporating dTTP into phage-specific DNAs in vitro. The synthesized DNAs were associated with proteins and sedimented with S values of 20, 50, and 90 in a sucrose gradient sedimentation. DNA isolated from 20S material was open circular replicative form (RF), DNA in 50S material was replicative-form DNA with an extended single-stranded viral DNA that ranged up to one genome in length, and DNA in 90S material consisted of circular and linear single-stranded viral DNA of full genome length and single-stranded viral DNA shorter than full genome length. Pulse and pulse-chase experiments indicated that 90S material derived from 50S material.

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References

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