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. 2020 Jul 16;11(7):451.
doi: 10.3390/insects11070451.

Molecular Identification and Geometric Morphometric Analysis of Haematobosca aberrans (Diptera: Muscidae)

Tanasak Changbunjong  1   2 , Jiraporn Ruangsittichai  3 , Gerard Duvallet  4 , Adrian C Pont  5
Affiliations

Molecular Identification and Geometric Morphometric Analysis of Haematobosca aberrans (Diptera: Muscidae)

Tanasak Changbunjong et al. Insects. .

Abstract

The genus Haematobosca Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020. In the present study, the mitochondrial cytochrome c oxidase I (COI) gene was used to confirm the morphological identification of H. aberrans. In addition, landmark-based geometric morphometrics was used to determine sexual dimorphism. The molecular analysis was conducted with 10 COI sequences. The results showed that all sequences were 100% identical. The sequence was not highly similar to reference sequences from GenBank and did not match any identified species from Barcode of Life Data Systems (BOLD). Phylogenetic analysis clearly differentiated this species from other species within the subfamily Stomoxyinae. For geometric morphometric analysis, a total of 16 wing pictures were analysed using the landmark-based approach. The results showed significant differences in wing shape between males and females, with a cross-validated classification score of 100%. The allometric analysis showed that wing shape has no correlation with size. Therefore, the COI gene is effective in species identification of H. aberrans, and geometric morphometrics is also effective in determining sexual dimorphism.

Keywords: Haematobosca; Stomoxyinae; Thailand; cytochrome c oxidase I; geometric morphometrics; sexual dimorphism.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Map showing the collection site of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020 in Thailand.
Figure 2
Figure 2
Ten landmarks digitised on wings of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020.
Figure 3
Figure 3
Neighbour-joining (NJ) tree based on Kimura two-parameter (K2P) model for cytochrome c oxidase I (COI) sequence of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020 collected from northern Thailand and sequences obtained from GenBank. Only bootstrap values ≥50% are shown. The scale bar represents 0.01% divergence.
Figure 4
Figure 4
Centroid size variation of the wings between sexes of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020, shown as quartile boxes. Each box shows the group median separating the 25th and 75th quartiles. Vertical bars under the boxes represent the wing (units as mm).
Figure 5
Figure 5
Configuration of the mean anatomical landmark positions connected by a straight line after Procrustes superimposition of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020 in both sexes.
Figure 6
Figure 6
Morphospace of males and females of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020 based on wing shape variables. The horizontal axis is the first relative warp (RW1), and the vertical axis is the second relative warp (RW2).
Figure 7
Figure 7
Factor map of the discriminant factor separating males (black bar) and females (grey bar) of Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020.
See this image and copyright information in PMC

References

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