Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Nov;98(6):476-482.
doi: 10.1002/cyto.b.21936. Epub 2020 Jul 27.

Determination of CD43 and CD200 surface expression improves accuracy of B-cell lymphoma immunophenotyping

Joerg Hoffmann  1 , Marissa Rother  1 , Ulrich Kaiser  2 , Michael C Thrun  3 , Christian Wilhelm  1 , Andrea Gruen  1 , Ute Niebergall  1 , Ute Meissauer  1 , Andreas Neubauer  1 , Cornelia Brendel  1
Affiliations

Determination of CD43 and CD200 surface expression improves accuracy of B-cell lymphoma immunophenotyping

Joerg Hoffmann et al. Cytometry B Clin Cytom. 2020 Nov.

Abstract

Background: The Matutes score (MS) was proposed to differentiate chronic lymphocytic leukemia (CLL) from other B-cell non-Hodgkin lymphomas (B-NHLs). However, ambiguous immunophenotypes are common and remain a diagnostic challenge. Therefore, we evaluated the diagnostic benefit of measuring CD200 and CD43 expression together with the standard MS antigens.

Methods: 138 lymphoma patient samples and a validation cohort of 138 additive samples were classified according to the standard MS and further assigned with one or two additional points, for high CD200 and/or CD43 expression levels. The "classical" MS and the "Matutes score-extended" (MS-e) were categorized as high (4-5/6-7), intermediate (2-3/4-5), and low (0-1/0-3). Samples were reclassified into the MS-e with focus on ambiguous cases with an intermediate "classical" MS.

Results: A total of 35 of 138 (25.4%) patient samples were assigned to the intermediate MS group and confirmed by histopathological reports as CLL (14/40.0%) and B-NHLs other than CLL (21/60%). MS-e analysis identified 13 of 14 (92.9%) of CLL cases (MS-e 4-5) and 18/21 (85.7%) non-CLL cases (MS-e ≤ 3) correctly. Overall, the sensitivity of the CLL diagnosis was significantly increased by application of MS-e compared to the "classical" MS (98.8% vs. 82.7%; p = 0.0009), while specificity of both methods was almost equal (94.7% vs. 98.3%; p = 0.4795). Of note, sole measurement of CD43 and CD200 on B-cells sufficiently differentiated CLL from non-CLL with a test accuracy superior to the "classical" MS (F1 score 96.2 vs. 93.6).

Conclusion: CD200 and CD43 have a high informative value in diagnostic immunophenotyping and facilitate the separation of CLL from other B-NHLs particularly in ambiguous cases.

Keywords: CD200; CD43; Matutes score; chronic lymphocytic leukemia; lymphoma.

PubMed Disclaimer

References

REFERENCES

    1. Alapat, D., Coviello-Malle, J., Owens, R., Qu, P., Barlogie, B., Shaughnessy, J. D., & Lorsbach, R. B. (2012). Diagnostic usefulness and prognostic impact of CD200 expression in lymphoid malignancies and plasma cell myeloma. American Journal of Clinical Pathology, 137, 93-100.
    1. Bettaieb, A., Farace, F., Mitjavila, M. T., Mishal, Z., Dokhelar, M. C., Tursz, T., … Kieffer, N. (1988). Use of a monoclonal antibody (GA3) to demonstrate lineage restricted O-glycosylation on leukosialin during terminal erythroid differentiation. Blood, 71, 1226-1233.
    1. Brunetti, L., Di Noto, R., Abate, G., Gorrese, M., Gravetti, A., Raia, M., … Del Vecchio, L. (2009). CD200/OX2, a cell surface molecule with immuno-regulatory function, is consistently expressed on hairy cell leukaemia neoplastic cells. British Journal of Haematology, 145, 665-667.
    1. Carlsson, S. R., & Fukuda, M. (1986). Isolation and characterization of leukosialin, a major sialoglycoprotein on human leukocytes. The Journal of Biological Chemistry, 261, 12779-12786.
    1. Challagundla, P., Medeiros, L. J., Kanagal-Shamanna, R., Miranda, R. N., & Jorgensen, J. L. (2014). Differential expression of CD200 in B-cell neoplasms by flow cytometry can assist in diagnosis, subclassification, and bone marrow staging. American Journal of Clinical Pathology, 142, 837-844.

MeSH terms