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. 2020 Jun;6(2):142-149.
doi: 10.5114/ceh.2020.95678. Epub 2020 May 27.

Glutathione S-transferase T1 and M1 null genotype distribution among non-alcoholic fatty liver disease patients and its association with cytokine and adipokine profiles

Vasyl Prysyazhnyuk  1 , Olexander Voloshyn  1 , Iryna Prysiazhniuk  1 , Tetiana Ilashchuk  1 , Larysa Sydorchuk  1 , Petro Prysyazhnyuk  1
Affiliations

Glutathione S-transferase T1 and M1 null genotype distribution among non-alcoholic fatty liver disease patients and its association with cytokine and adipokine profiles

Vasyl Prysyazhnyuk et al. Clin Exp Hepatol. 2020 Jun.

Abstract

Aim of the study: Among the key genes involved in the development of non-alcoholic fatty liver disease (NAFLD) are genes encoding the synthesis of glutathione S-transferase (GST).

Material and methods: Deletion polymorphism of GSTT1 and GSTM1 genes was investigated in 104 NAFLD patients and 45 healthy individuals. Biochemical blood analysis, tumor necrosis factor-α (TNF-α), interleukin-10, leptin and adiponectin plasma levels were studied.

Results: The distribution of deletion vs. non-deletion genotypes of the GSTT1 gene in NAFLD patients was 18 (17.3%) vs. 86 (82.7%) patients and in healthy people it was 6 (13.3%) vs. 39 (86.7%) individuals. The genotype distribution of the GSTM1 gene was as follows: 52 (50.0%) NAFLD patients had null genotype vs. 52 patients (50.0%) with non-deletion genotype; in the control group - 23 (51.1%) vs. 22 (48.9%) individuals. Deletion of the GSTT1 gene in NAFLD patients was associated with twice as high (p = 0.01) TNF-α level in the blood as compared to patients with normal genotype. Higher concentration of leptin in blood by 37.1% (p = 0.04) was observed in patients with null genotype of the GSTM1 gene, as compared to those with normal genotype.

Conclusions: Deletion polymorphism of GSTT1 and GSTM1 genes distribution among NAFLD patients did not differ as compared to healthy individuals. Null-genotype GSTT1 gene carriers were characterized by higher TNF-α concentration and null-genotype GSTM1 gene carriers were characterized by elevated leptin level as compared to normal genotype carriers.

Keywords: adiponectin; glutathione S-transferase gene; leptin; non-alcoholic fatty liver disease.

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Conflict of interest statement

The authors report no conflict of interest.

Figures

Fig. 1
Fig. 1
Electropherogram of the amplified GSTT1 and GSTM1 gene fragments’ distribution. Example 1 – negative control, examples 2, 5 – del GSTT1/del GSTM1 genotype; example 3 – allele GSTT1/del GSTM1 genotype; examples 4, 6-8, 10-13, 15 – allele GSTT1/allele GSTM1 genotype, M – molecular gene ruler
See this image and copyright information in PMC

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