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. 2020 Oct 2;15(10):1798108.
doi: 10.1080/15592324.2020.1798108. Epub 2020 Jul 30.

Physiological and proteomics responses of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism in mulberry (Morus alba L.) leaves to NaCl and NaHCO3 stress

Huihui Zhang  1   2 , Yuze Huo  1 , Zisong Xu  1 , Kaiwen Guo  1 , Yue Wang  2 , Xiaosong Zhang  3 , Nan Xu  2   4   5 , Guangyu Sun  2
Affiliations

Physiological and proteomics responses of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism in mulberry (Morus alba L.) leaves to NaCl and NaHCO3 stress

Huihui Zhang et al. Plant Signal Behav. .

Abstract

In order to find out the response mechanism of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism in mulberry (Morus alba L.) leaves under NaCl and NaHCO3 stress, and to reveal its role in salt alkali adaptation. The effects of the nitrogen metabolism of mulberry leaves were studied under 100 mmol L-1 NaCl and NaHCO3 stress.The results showed that the activity of NR and the content of TN and SP did not change significantly, the expression of NiR, Fd-NiR, Fd-NiR gene and theactivity of NiR increased significantly under NaCl stress, but nitrogen assimilation was inhibited under NaHCO3 stress. NaCl stress had no significant effect on the expression and activity of GS and GOGAT in mulberry leaves. Under NaHCO3 stress, the expression of Fd-GOGAT, Fd-GOGAT2, Fd-GOGAT gene, and the activity of GS and GOGAT were significantly decreased. NaCl stress can promote the accumulation of Pro, Put and Spd in mulberry leaves. The accumulation of Pro under NaHCO3 stress is greater than that under NaCl stress. NaCl stress also induced the up-regulation of GAD, GAD1 and GAD1 gene expression, so promoting the synthesis of GABA may be an adaptive mechanism for mulberry to cope with NaCl stress, but the expression of GAD did not change significantly and GAD gene expression lower than CK under NaHCO3 stress. Although both NaCl and NaHCO3 stress could promote the synthesis of GSH by up-regulation of GCLM expression, GSH under NaHCO3 stress was significantly higher than that under NaCl stress, the content of H2O2 was still significantly higher than that of NaCl stress, that means GSH may not play a key role in alleviating the oxidative damage in mulberry leaves caused by salt and alkali.

Keywords: Mulberry; nitrogen metabolism; polyamines; proline; proteomics; salt and alkali.

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Figures

Figure 1.
Figure 1.
TN content (a), SP content (b), NR activity (c) and NiR activity (d) of mulberry leaves under NaCl and NaHCO3 stress. Note: The data are from three replicated experiments (n = 3), and represent means ± SE. Significant differences were expressed by different small letters (P < .05), and very significant differences were expressed by different capital letters (P < .01).
Figure 2.
Figure 2.
GOGAT activity (a) and GS activity (b) of mulberry leaves under NaCl and NaHCO3 stress. Note: The data are from three replicated experiments (n = 3), and represent means ± SE. Significant differences were expressed by different small letters (P < .05), and very significant differences were expressed by different capital letters (P < .01).
Figure 3.
Figure 3.
Pro content (a), Put content (b), Spd content (c), Spm content (d),GSH content (e) and H2O2 content (f) of mulberry leaves under NaCl and NaHCO3 stress. Note: The data are from three replicated experiments (n = 3), and represent means ± SE. Significant differences were expressed by different small letters (P < .05), and very significant differences were expressed by different capital letters (P < .01).
Figure 4.
Figure 4.
Effects of NaCl and NaHCO3 stress on gene expression of nitrogen assimilation (a) and glutamine/glutamine family of amino acids metabolism (b) in leaves of mulberry seedlings. Note: * indicates significant difference with CK.
Figure 5.
Figure 5.
Schematic presentation of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism of mulberry leaves under NaCl and NaHCO3 stress.
See this image and copyright information in PMC

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