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. 2020 Jul 28;10(8):1477.
doi: 10.3390/nano10081477.

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity

Irina A Shurygina  1 , Galina F Prozorova  2 , Irina S Trukhan  1 , Svetlana A Korzhova  2 , Tatiana V Fadeeva  1 , Alexander S Pozdnyakov  2 , Nataliya N Dremina  1 , Artem I Emel'yanov  2 , Nadezhda P Kuznetsova  2 , Michael G Shurygin  1
Affiliations

NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity

Irina A Shurygina et al. Nanomaterials (Basel). .

Abstract

Novel silver/poly-1-vinyl-1,2,4-triazole nanocomposite materials-possessing antimicrobial activity against Gram-positive and Gram-negative bacteria-have been synthesized and characterized in the solid state and aqueous solution by complex of modern physical-chemical and biologic methods. TEM-monitoring has revealed the main stages of microbial cell (E. coli) destruction by novel nanocomposite. The concept of direct polarized destruction of microbes by nanosilver proposed by the authors allows the relationship between physicochemical and antimicrobial properties of novel nanocomposites. At the same time, it was shown that the nanocomposite was nontoxic to the fibroblast cell culture. Thus, the synthesized nanocomposite combining antibacterial activity against Gram-positive and Gram-negative bacteria as well as the absence of toxic effects on mammalian cells is a promising material for the development of catheters, coatings for medical devices.

Keywords: antimicrobial activity; nontoxic nanocomposite; poly-1-vinyl-1,2,4-triazole; silver nanoparticles.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Scheme 1
Scheme 1
Synthesis of poly-1-vinyl-1,2,4-triazole (PVT).
Figure 1
Figure 1
(a) SEM image of PVT and (b) particle size distribution curves of the (1) initial PVT and (2) AgNPs nanocomposite.
Figure 2
Figure 2
Schematic coordination interaction of silver ions with PVT macromolecules.
Figure 3
Figure 3
Formation of macromolecular globules during the incorporation of AgNPs into a polymer matrix.
Figure 4
Figure 4
(a) UV-visible spectra of AgNPs nanocomposites synthesized using various different reducing agents: NaBH4 (1) and formaldehyde (2); (b) typical X-ray diffraction pattern of AgNPs nanocomposites.
Figure 5
Figure 5
(a,c) TEM images and (b,d) sizes histograms of AgNPs nanocomposites synthesized using various different reducing agents. (a,b) NaBH4 and (c,d) formaldehyde.
Figure 6
Figure 6
TEM images of the interaction of AgNPs nanocomposites with E. coli after (A–C) 1 h, (D) 2 h and (E) 24 h incubation, (F) control. Black arrows—bacteria, white arrows—nanocomposite.
Figure 7
Figure 7
Incubation with fibroblasts for 24 h: control, PVT 16 µg/mL, AgNPs nanocomposites (PVT-Ag) 16 µg/mL. Phase contrast, fluorescence staining with fluorescein diacetate (FDA) (green) and propidium iodide (PI) (red). Scale bars 100 µm.
Figure 8
Figure 8
Analysis of the number of dead fibroblasts after incubation for 24 h with (A) PVT, (B) Ag(0)-PVT in the different concentrations and (C) in comparison to each other.. Medians, first and third quartiles are presented; p-values are given only for significant differences (p < 0.05).
See this image and copyright information in PMC

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