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. 2021 Feb;130(2):504-515.
doi: 10.1111/jam.14802. Epub 2020 Aug 14.

Survival and infectivity of Toxoplasma gondii and Cryptosporidium parvum oocysts bioaccumulated by Dreissena polymorpha

E Géba  1   2 , A Rousseau  2   3 , A Le Guernic  1 , S Escotte-Binet  2 , L Favennec  4 , S La Carbona  3 , G Gargala  4 , J P Dubey  5 , I Villena  2 , S Betoulle  1 , D Aubert  2 , A Bigot-Clivot  1
Affiliations

Survival and infectivity of Toxoplasma gondii and Cryptosporidium parvum oocysts bioaccumulated by Dreissena polymorpha

E Géba et al. J Appl Microbiol. 2021 Feb.

Abstract

Aims: The study was aimed to understand the depuration process of Cryptosporidium parvum and Toxoplasma gondii oocysts by zebra mussel (Dreissena polymorpha), to consider the use of the zebra mussel as a bioremediation tool.

Materials and methods: Two experiments were performed: (i) individual exposure of mussel to investigate oocyst transfers between bivalves and water and (ii) in vivo exposure to assess the ability of the zebra mussel to degrade oocysts.

Results: (i) Our results highlighted a transfer of oocysts from the mussels to the water after 3 and 7 days of depuration; however, some oocysts were still bioaccumulated in mussel tissue. (ii) Between 7 days of exposure at 1000 or 10 000 oocysts/mussel/day and 7 days of depuration, the number of bioaccumulated oocysts did not vary but the number of infectious oocysts decreased.

Conclusion: Results show that D. polymorpha can release oocysts in water via (pseudo)faeces in depuration period. Oocysts remain bioaccumulated and infectious oocyst number decreases during the depuration period in zebra mussel tissues. Results suggest a degradation of bioaccumulated C. parvum and T. gondii oocysts.

Significance and impact of the study: This study highlighted the potential use of D. polymorpha as a bioremediation tool to mitigate of protozoan contamination in water resources.

Keywords: CC-qPCR; bioassay; bioremediation; infectious protozoa; zebra mussel.

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