Resourceful treatment of cane sugar industry wastewater by Tribonema minus towards the production of valuable biomass

Abstract

Tribonema minus was cultivated in different concentrations of sugarcane wastewater (SW) diluted with mBG-11 medium to produce biomass for biodiesel, bioproduct, and biomaterial production. The results showed that T. minus grew mixotrophically in 50%SW, with the highest biomass accumulation (7.86 g/L) and nutrient removal efficiency (84.85% of nitrogen, 62.57% of phosphorus, and 44.72% of COD). Excluding 100%SW, the chrysolaminarin and cellulose contents increased with increasing SW concentration; the highest contents of 8.11% and 25.69% dry weight were reached in 75%SW, respectively. Although fewer lipids and palmitoleic acid accumulated at higher SW concentrations, their productivities were significantly higher than those in the control due to the higher contribution of biomass. Moreover, the fatty acid profiles produced at the tested concentrations showed superior biodiesel properties. These findings suggested that the addition of mBG-11 medium to SW might be an effective strategy for valuable biomass production in T. minus and SW bioremediation.

Keywords: Bioremediation; Sugarcane wastewater; Tribonema minus; Valuable biomass production; Value-added biochemicals.