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. 2020 May;23(5):654-662.
doi: 10.22038/IJBMS.2020.40915.9695.

Relationship between calcium circulation-related factors and muscle strength in rat sciatic nerve injury model

Xiaoming Sun  1 , Wei Wang  1 , Yangyi Dong  1 , Yue Wang  1 , Meixiang Zhang  1 , Zhao Wang  1 , Xiaowei Yu  1 , Jiao Huang  1 , Hongxing Cai  1
Affiliations

Relationship between calcium circulation-related factors and muscle strength in rat sciatic nerve injury model

Xiaoming Sun et al. Iran J Basic Med Sci. 2020 May.

Abstract

Objectives: The purpose of this study is to investigate the indication function of the calcium circulation-related factors on the damage to muscle strength and contraction function after nerve injury. The target factors include ryanodine receptor (RyR), inositol-1,4,5-triphosphate receptor (IP3R), phospholamban (PLN), cryptocalcitonin (CASQ), ATPase and troponin C (TNNC).

Materials and methods: Sprague-Dawley (SD) rats were randomly divided into sham-operated group (SO), sciatic nerve injury group (SNI) and sciatic nerve disconnection group (SNT). Sciatic nerve function index and stretching test were used to examine the changes to muscle strength; bilateral gastrocnemius muscles were extracted after execution for gastrocnemius wet weight ratio test. HE staining slides and average cross-sectional area of muscle fibers were acquired to analyze the muscle atrophy. The transcription level of the factors was also measured.

Results: Sciatic nerve damage in SNI group was significantly higher than that in SO group in the 6 weeks, but there was no significant difference between SNT and SO groups fallowing sciatic nerve damage. Sciatic nerve function in SNT group was worse than that in SNI group. The average cross-sectional area of gastrocnemius muscle fibers in SNI and SNT groups was significantly reduced compared to that in SO group. The transcriptional levels of RyR, PLN, CASQ, ATPase and TNNC in SNI and SNT groups were significantly different from those in SO group.

Conclusion: Calcium circulation-related factors could be used as potential indicators for assessment of damages to muscle strength.

Keywords: Forensic medicine; Muscle atrophy; Muscle strength; Ryanodine receptor; Sciatic nerve.

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Figures

Figure 1
Figure 1
Gastrocnemius muscle wet weight ratio of rats
Figure 2
Figure 2
Sciatic nerve function index of rats
Figure 3
Figure 3
Functional defect ratio of rats
Figure 4
Figure 4
Gastrocnemius muscle fiber cross-sectional area of rats
Figure 5.
Figure 5.
HE staining of gastrocnemius muscle cross section on the operative side of rats (100 times)
Figure 6
Figure 6
Transcription level of RyR1 in rat gastrocnemius muscle
Figure 7
Figure 7
Transcription level of PLN in rat gastrocnemius muscle
Figure 8
Figure 8
Transcription level of CASQ in rat gastrocnemius muscle
Figure 9
Figure 9
Transcription level of ATPase in rat gastrocnemius muscle
Figure 10
Figure 10
Transcription level of TNNC in rat gastrocnemius muscle
Figure 11
Figure 11
The transcription level of calcium cycle related genes in rat gastrocnemius muscle and the lower limb muscle function ratio in SNI group
See this image and copyright information in PMC

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