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. 2020 Aug 3;13(1):394.
doi: 10.1186/s13071-020-04268-y.

Laboratory colonization and maintenance of Anopheles atroparvus from the Ebro Delta, Spain

Lotty Birnberg  1 , Carles Aranda  1   2 , Sandra Talavera  1 , Ana I Núñez  1 , Raúl Escosa  3 , Núria Busquets  4
Affiliations

Laboratory colonization and maintenance of Anopheles atroparvus from the Ebro Delta, Spain

Lotty Birnberg et al. Parasit Vectors. .

Abstract

Background: Historically, Anopheles atroparvus has been considered one of the most important malaria vectors in Europe. Since malaria was eradicated from the European continent, the interest in studying its vectors reduced significantly. Currently, to better assess the potential risk of malaria resurgence on the continent, there is a growing need to update the data on susceptibility of indigenous Anopheles populations to imported Plasmodium species. In order to do this, as a first step, an adequate laboratory colony of An. atroparvus is needed.

Methods: Anopheles atroparvus mosquitoes were captured in rice fields from the Ebro Delta (Spain). Field-caught specimens were maintained in the laboratory under simulated field-summer conditions. Adult females were artificially blood-fed on fresh whole rabbit blood for oviposition. First- to fourth-instar larvae were fed on pulverized fish and turtle food. Adults were maintained with a 10% sucrose solution ad libitum.

Results: An An. atroparvus population from the Ebro Delta was successfully established in the laboratory. During the colonization process, feeding and hatching rates increased, while a reduction in larval mortality rate was observed.

Conclusions: The present study provides a detailed rearing and maintenance protocol for An. atroparvus and a publicly available reference mosquito strain within the INFRAVEC2 project for further research studies involving vector-parasite interactions.

Keywords: Anopheles atroparvus; Colonization; Europe; Malaria.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Development of Anopheles atroparvus collected from the Ebro Delta under controlled laboratory conditions. a Feeding rate (FR), engorged females/total number of females at the time of feeding. b Hatching rate (HR), total number of L1/total number of eggs. c Larval mortality rate (LMR), total number of pupae/total number of L1
See this image and copyright information in PMC

References

    1. Sinka ME, Bangs MJ, Manguin S, Coetzee M, Mbogo CM, Hemingway J. et al. The dominant Anopheles vectors of human malaria in Africa, Europe and the Middle East: occurrence data, distribution and bionomic précis. Parasit Vectors. 2010;3:117. - PMC - PubMed
    1. Linton YM, Smith L, Koliopoulos G, Zounos AK, Samanidou-Voyadjoglou A, Patsoula E. The Anopheles (Anopheles) maculipennis complex (Diptera: Culicidae) in Greece. J Nat His. 2007;41:2683–2699. doi: 10.1080/00222930701403255. - DOI
    1. Harbach RE. The phylogeny and classification of Anopheles. In: Manguin S, editor. Anopheles mosquitoes - new insights into malaria vectors. London, UK: IntechOpen; 2013. pp. 4–55.
    1. Hertig E. Distribution of Anopheles vectors and potential malaria transmission stability in Europe and the Mediterranean area under future climate change. Parasit Vectors. 2019;12:18. doi: 10.1186/s13071-018-3278-6. - DOI - PMC - PubMed
    1. Piperaki ET, Daikos GL. Malaria in Europe: emerging threat or minor nuisance? Clin Microbiol Infect. 2016;22:6. - PubMed

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