Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Nov;44(11):2263-2274.
doi: 10.1002/cbin.11434. Epub 2020 Aug 10.

Long noncoding RNA ERICD interacts with ARID3A via E2F1 and regulates migration and proliferation of osteosarcoma cells

Kaifee Arman  1   2   3 , Khandakar A S M Saadat  3 , Yusuf Z Igci  4 , Esra Bozgeyik  5 , Masa-Aki Ikeda  6 , Ecir A Cakmak  7 , Ahmet Arslan  8
Affiliations

Long noncoding RNA ERICD interacts with ARID3A via E2F1 and regulates migration and proliferation of osteosarcoma cells

Kaifee Arman et al. Cell Biol Int. 2020 Nov.

Abstract

Long noncoding RNA (lncRNA) dysregulation is known to be taking part in majority of cancers, including osteosarcoma. In one of our previous studies, we showed that lncRNA MEG3 is being regulated by microRNA-664a (miR-664a) suppresses the migratory potential of osteosarcoma cells (U-2OS). We now report a novel lncRNA, namely, ERICD, which is linked to the transcription factor AT-rich interaction domain 3A (ARID3A) in U-2OS cells. We show that ARID3A binds to ERICD and indirectly interacts with each other via the E2F transcription factor 1 (E2F1). Furthermore, small interfering RNA (siRNA)-mediated knockdown of ERICD inhibited cell migration, formation of colonies, and proliferation in U-2OS cells. Overexpression of ARID3A inhibited cell migration, colony formation, and proliferation, whereas siRNA-mediated knockdown of ARID3A promoted cell migration, colony formation, and proliferation. Our findings indicate that ARID3A and lncRNA ERICD have plausible tumor suppressive and oncogenic functions, respectively, in osteosarcoma. Our data demonstrate the converse interaction between ARID3A and lncRNA ERICD that target DNA-binding proteins and dysregulation of their expression through E2F1 augments osteosarcoma progression. The cell rescue experiment also indicated E2F1 to be involved in the regulation of ARID3A and ERICD.

Keywords: ARID3A; DNA-binding lncRNA; colony formation; lncRNA ERICD; migration; osteosarcoma.

PubMed Disclaimer

References

REFERENCES

    1. Alvarez-Dominguez, J. R., Knoll, M., Gromatzky, A. A., & Lodish, H. F. (2017). The super-enhancer-derived alncRNA-EC7/Bloodlinc potentiates red blood cell development in trans. Cell Reports, 19(12), 2503-2514. https://doi.org/10.1016/j.celrep.2017.05.082
    1. Alvarez-Dominguez, J. R., & Lodish, H. F. (2017). Emerging mechanisms of long noncoding RNA function during normal and malignant hematopoiesis. Blood, 130(18), 1965-1975. https://doi.org/10.1182/blood-2017-06-788695
    1. Bobbs, A., Gellerman, K., Hallas, W. M., Joseph, S., Yang, C., Kurkewich, J., & Cowden Dahl, K. D. (2015). ARID3B directly regulates ovarian cancer promoting genes. PLOS One, 10(6), e0131961. https://doi.org/10.1371/journal.pone.0131961
    1. Bousquet, M., Noirot, C., Accadbled, F., Sales de Gauzy, J., Castex, M. P., Brousset, P., & Gomez-Brouchet, A. (2016). Whole-exome sequencing in osteosarcoma reveals important heterogeneity of genetic alterations. Annals of Oncology, 27(4), 738-744. https://doi.org/10.1093/annonc/mdw009
    1. Bozgeyik, E., Igci, Y. Z., Sami Jacksi, M. F., Arman, K., Gurses, S. A., Bozgeyik, I., … Igci, M. (2016). A novel variable exonic region and differential expression of LINC00663 non-coding RNA in various cancer cell lines and normal human tissue samples. Tumour Biology, 37(7), 8791-8798. https://doi.org/10.1007/s13277-015-4782-3

MeSH terms

LinkOut - more resources