Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

doi:10.3390/ijerph17155508

. 2020 Jul 30;17(15):5508.

doi: 10.3390/ijerph17155508.

Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

Hana Mlejnkova¹, Katerina Sovova², Petra Vasickova³, Vera Ocenaskova¹, Lucie Jasikova¹, Eva Juranova¹

Affiliations

¹ T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic.
² T. G. Masaryk Water Research Institute, Public Research Institution, Brno Branch, Mojmirovo namesti 16, 612 00 Brno, Czech Republic.
³ Veterinary Research Institute, Public Research Institution, Hudcova 296/70, 621 00 Brno, Czech Republic.

PMID: 32751749
PMCID: PMC7432771
DOI: 10.3390/ijerph17155508

Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

Hana Mlejnkova et al. Int J Environ Res Public Health. 2020.

. 2020 Jul 30;17(15):5508.

doi: 10.3390/ijerph17155508.

Authors

Hana Mlejnkova¹, Katerina Sovova², Petra Vasickova³, Vera Ocenaskova¹, Lucie Jasikova¹, Eva Juranova¹

Affiliations

¹ T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic.
² T. G. Masaryk Water Research Institute, Public Research Institution, Brno Branch, Mojmirovo namesti 16, 612 00 Brno, Czech Republic.
³ Veterinary Research Institute, Public Research Institution, Hudcova 296/70, 621 00 Brno, Czech Republic.

PMID: 32751749
PMCID: PMC7432771
DOI: 10.3390/ijerph17155508

Abstract

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.

Keywords: RT-qPCR; SARS-CoV-2; coronavirus; early warning system; epidemic; virus; wastewater; wastewater-based epidemiology.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

**Figure 1**
Regions of the Czech Republic where SARS-CoV-2 RNA was detected in wastewater.

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[1] Drexler J.F., Corman M.V., Drosten C. Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS. Antivir. Res. 2014;101:45–56. doi: 10.1016/j.antiviral.2013.10.013. - DOI - PMC - PubMed

[2] Drexler J.F., Corman M.V., Drosten C. Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS. Antivir. Res. 2014;101:45–56. doi: 10.1016/j.antiviral.2013.10.013. - DOI - PMC - PubMed

[3] Chan J.F.W., Lau S.K.P., To K.K.W., Cheng V.C.C., Woo P.C.Y., Yuen K.Y. Middle east respiratory syndrome coronavirus: Another zoonotic betacoronavirus casuing SARS-like disease. Clin. Microbiol. Rev. 2015;28:465–522. doi: 10.1128/CMR.00102-14. - DOI - PMC - PubMed

[4] Chan J.F.W., Lau S.K.P., To K.K.W., Cheng V.C.C., Woo P.C.Y., Yuen K.Y. Middle east respiratory syndrome coronavirus: Another zoonotic betacoronavirus casuing SARS-like disease. Clin. Microbiol. Rev. 2015;28:465–522. doi: 10.1128/CMR.00102-14. - DOI - PMC - PubMed

[5] Chan P.K., Chan M.C. Tracing the SARS-coronavirus. J. Thorac. Dis. 2013;5:S118–S121. doi: 10.3978/j.issn.2072-1439.2013.06.19. - DOI - PMC - PubMed

[6] Chan P.K., Chan M.C. Tracing the SARS-coronavirus. J. Thorac. Dis. 2013;5:S118–S121. doi: 10.3978/j.issn.2072-1439.2013.06.19. - DOI - PMC - PubMed

[7] Ding Y., He L., Zhang Q., Huang Z., Che X., Hou J., Wang H., Shen H., Qiu L., Li Z., et al. Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients: Implications for pathogenesis and virus transmission pathways. J. Pathol. 2004;203:622–630. doi: 10.1002/path.1560. - DOI - PMC - PubMed

[8] Ding Y., He L., Zhang Q., Huang Z., Che X., Hou J., Wang H., Shen H., Qiu L., Li Z., et al. Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients: Implications for pathogenesis and virus transmission pathways. J. Pathol. 2004;203:622–630. doi: 10.1002/path.1560. - DOI - PMC - PubMed

[9] Su S., Wong G., Shi W., Liu J., Lai A.C.K., Zhou J., Liu W., Bi Y., Gao G.F. Epidemiology, genetic, recombination, and pathogenesis of coronaviruses. Trends Microbiol. 2016;24:490–502. doi: 10.1016/j.tim.2016.03.003. - DOI - PMC - PubMed

[10] Su S., Wong G., Shi W., Liu J., Lai A.C.K., Zhou J., Liu W., Bi Y., Gao G.F. Epidemiology, genetic, recombination, and pathogenesis of coronaviruses. Trends Microbiol. 2016;24:490–502. doi: 10.1016/j.tim.2016.03.003. - DOI - PMC - PubMed

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Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

Affiliations

Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

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Cited by

References

Publication types

MeSH terms

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Grants and funding

LinkOut - more resources

Full Text Sources

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