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. 2020 Jul 20;35(4):e150.
doi: 10.5001/omj.2020.69. eCollection 2020 Jul.

Association of rs12487066, rs12044852, rs10735781, rs3135388, rs6897932, rs1321172, rs10492972, and rs9657904 Polymorphisms with Multiple Sclerosis in Iranian Population

Takavar Razavian  1 , Mahdieh Ebrahimi Shakib  2 , Kurosh Gharagozli  3 , Hossein Maghsoudi  4 , Seyed Kazem Bidoki  5 , Soha Sadeghi  6 , Massoud Houshmand  7   8
Affiliations

Association of rs12487066, rs12044852, rs10735781, rs3135388, rs6897932, rs1321172, rs10492972, and rs9657904 Polymorphisms with Multiple Sclerosis in Iranian Population

Takavar Razavian et al. Oman Med J. .

Abstract

Objectives: Multiple sclerosis (MS) is a chronic disease of the central nervous system. The pathogenesis of MS is best described by a multifactorial model incorporating interactions between genetic and environmental factors with the role of genetic factors increasingly taken into account. The main goal of this study was to investigate the associations of rs12487066, rs12044852, rs10735781, rs3135388, rs6897932, rs1321172, rs10492972, and rs9657904 polymorphisms with MS in the Iranian population.

Methods: A total of 83 patients with MS (82.0% female and 18.0% male; mean age = 35.2±8.6 years) and 100 physically and mentally healthy subjects (81.0% female and 19.0% male; mean age = 40.4±6.4 years) were selected using convenient sampling. A 5 mL blood sample was taken from each case and control patient. We used the tetra-primer ARMS-PCR method to genotype the desired polymorphisms. The associations between polymorphisms and the disease were studied based on codominant, dominant, recessive, and overdominant models.

Results: The rs10735781 polymorphism was codominantly (p = 0.029), overdominantly (p = 0.008), and dominantly (p = 0.009) associated with the disease. The rs6897932 was also found to be codominantly (p = 0.012), dominantly (p = 0.019), and recessively (p = 0.011) associated with the disease.

Conclusions: We found an association between the rs10735781 and rs6897932 polymorphisms on the EVI5 and IL7RA genes, respectively, with increased MS in the Iranian population. Therefore, single nucleotide polymorphisms in the EVI5 and IL7RA genes can be considered a prognostic marker of MS.

Keywords: Genotype; Iran; Multiple Sclerosis; Polymerase Chain Reaction; Polymorphism, Single Nucleotide.

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Figures

Figure 1
Figure 1
The mean frequency of symptoms associated with multiple sclerosis in patients.
Figure 2
Figure 2
The amplified sequences of (a) rs10735781 and (b) rs6897932 through the tetra-primer amplification-refractory mutation system in conjunction with polymerase chain reaction method.
Figure 3
Figure 3
Image of gene sequencing KIF1B gene (rs10492972). This image related to homozygous normal genotype (TT).
See this image and copyright information in PMC

References

    1. Baranzini SE, Oksenberg JR. The genetics of multiple sclerosis: from 0 to 200 in 50 years. Trends Genet 2017. Dec;33(12):960-970. 10.1016/j.tig.2017.09.004 - DOI - PMC - PubMed
    1. Jafarzadeh A, Mahdavi R, Jamali M, Hajghani H, Nemati M, Ebrahimi H-A. Increased concentrations of interleukin-33 in the serum and cerebrospinal fluid of patients with multiple sclerosis. Oman Med J 2016. Jan;31(1):40-45. 10.5001/omj.2016.08 - DOI - PMC - PubMed
    1. Kister I, Bacon TE, Chamot E, Salter AR, Cutter GR, Kalina JT, et al. Natural history of multiple sclerosis symptoms. Int J MS Care 2013;15(3):146-158. 10.7224/1537-2073.2012-053 - DOI - PMC - PubMed
    1. Chaudhuri A, Behan PO. Multiple sclerosis is not an autoimmune disease. Arch Neurol 2004. Oct;61(10):1610-1612. 10.1001/archneur.61.10.1610 - DOI - PubMed
    1. Hauser SL, Oksenberg JR. The neurobiology of multiple sclerosis: genes, inflammation, and neurodegeneration. Neuron 2006. Oct;52(1):61-76. 10.1016/j.neuron.2006.09.011 - DOI - PubMed

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