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Clinical Trial
. 2020 Aug 6;13(1):372.
doi: 10.1186/s13104-020-05212-0.

COVID19 antibody detection using lateral flow assay tests in a cohort of convalescent plasma donors

Brett Ragnesola  1 , Daniel Jin  1 , Christopher C Lamb  2   3   4 , Beth H Shaz  1 , Christopher D Hillyer  1 , Larry L Luchsinger  5
Affiliations
Clinical Trial

COVID19 antibody detection using lateral flow assay tests in a cohort of convalescent plasma donors

Brett Ragnesola et al. BMC Res Notes. .

Abstract

Objective: COVID19 has caused a global and ongoing pandemic. The need for population seroconversion data is apparent to monitor and respond to the pandemic. Using a lateral flow assay (LFA) testing platform, the seropositivity in 63 New York Blood Center (NYBC) Convelescent Plasma (CP) donor samples were evaluated for the presence of COVID19 specific IgG and IgM.

Results: CP donors showed diverse antibody result. Convalescent donor plasma contains SARS-CoV-2 specific antibodies. Weak antibody bands may identify low titer CP donors. LFA tests can identify antibody positive individuals that have recovered from COVID19. Confirming suspected cases using antibody detection could help inform the patient and the community as to the relative risk to future exposure and a better understanding of disease exposure.

Keywords: Antibody testing; Convalescent plasma; Covid-19.

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Conflict of interest statement

CL worked with the LFD manufacturer on the Emergency Use Authorization submission to the US FDA.

Figures

Fig. 1
Fig. 1
a Procedural schematic for CLUNGENE® Immunoassay. One drop is equal to ~ 20 uL. b Visual interpretation guide for assays. c Representative convalescent donor plasma (CP) or frozen fresh plasma (FFP) assay result images
Fig. 2
Fig. 2
a Frequency of IgG assay results from CP donor samples. b Frequency of IgM assay results from CP donor samples. c Overall CP donor test result. d Frequency of assay result duplication using identical CP donor samples
See this image and copyright information in PMC

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