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. 2020 Aug 7;15(8):e0237134.
doi: 10.1371/journal.pone.0237134. eCollection 2020.

Identification of chemosensory genes from the antennal transcriptome of Semiothisa cinerearia

Panjing Liu  1 , Xiaofang Zhang  1 , Runjie Meng  2 , Chang Liu  3 , Min Li  1 , Tao Zhang  1
Affiliations

Identification of chemosensory genes from the antennal transcriptome of Semiothisa cinerearia

Panjing Liu et al. PLoS One. .

Abstract

Olfaction plays vital roles in the survival and reproduction of insects. The completion of olfactory recognition requires the participation of various complex protein families. However, little is known about the olfactory-related proteins in Semiothisa cinerearia Bremer et Grey, an important pest of Chinese scholar tree. In this study, we sequenced the antennal transcriptome of S. cinerearia and identified 125 olfactory-related genes, including 25 odorant-binding proteins (OBPs), 15 chemosensory proteins (CSPs), two sensory neuron membrane proteins (SNMPs), 52 odorant receptors (ORs), eight gustatory receptors (GRs) and 23 ionotropic receptors (IRs). BLASTX best hit results and phylogenetic analyses indicated that these genes were most identical to their respective orthologs from Ectropis obliqua. Further quantitative real-time PCR (qRT-PCR) analysis revealed that three ScinOBPs and three ScinORs were highly expressed in male antennae, while seven ScinOBPs and twelve ScinORs were female-specifically expressed. Our study will be useful for the elucidation of olfactory mechanisms in S. cinerearia.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Phylogenetic tree of OBPs in Semiothisa cinerearia, Ectropis obliqua, Bombyx mori and Helicoverpa armigera.
The sequences used in this analysis are listed in S4 Table.
Fig 2
Fig 2. Amino acid sequence alignment of classic-OBPs in Semiothisa cinerearia and Ectropis obliqua.
Fig 3
Fig 3. Expression of Semiothisa cinerearia OBPs (A), CSPs and SNMPs (B) by qRT-PCR.
* indicates significant difference between female and male at p < 0.05, and ** indicates significant difference at p < 0.01.
Fig 4
Fig 4. Phylogenetic tree of CSP proteins.
Sequences used were from Hymenoptera (Solenopsis invicta), Coleoptera (Tribolium castaneum), Diptera (Drosophila melanogaster), and Lepidoptera (Bombyx mori, Helicoverpa armigera, Heliothis virescens, Mamestra brassicae, Manduca sexta, Plutella xylostella, Ectropis obliqua, Spodoptera exigua, Ostrinia furnacalis). The sequences listed in S5 Table.
Fig 5
Fig 5. Phylogenetic tree of SNMP proteins in Semiothisa cinerearia, Ectropis obliqua, Spodoptera exigua, Spodoptera litura, Bombyx mori, Ostrinia furnacalis, Helicoverpa armigera and Heliothis virescens.
The sequences used in this analysis are listed in S6 Table.
Fig 6
Fig 6. Phylogenetic tree of OR proteins in Semiothisa cinerearia, Ectropis obliqua, Chilo suppressalis, Bombyx mori and Helicoverpa armigera.
The sequences used in this analysis are listed in S7 Table.
Fig 7
Fig 7. Phylogenetic tree of GR proteins in Semiothisa cinerearia, Ectropis obliqua, Bombyx mori, Ostrinia furnacalis, Helicoverpa armigera and Drosophila melanogaster.
The sequences used in this analysis are listed in S8 Table.
Fig 8
Fig 8. Phylogenetic tree of IR proteins in Semiothisa cinerearia, Ectropis obliqua, Spodoptera litura, Ostrinia furnacalis, Bombyx mori and Drosophila melanogaster.
The sequences used in this analysis are listed in S9 Table.
Fig 9
Fig 9. Expression of Semiothisa cinerearia ORs (A), GRs and IRs (B) by qRT-PCR.
* indicates significant difference between female and male at p <0.05, and ** indicates significant difference at p < 0.01.
See this image and copyright information in PMC

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