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Case Reports
. 2020 Jul;33(3):177-181.
doi: 10.1293/tox.2019-0081. Epub 2020 Mar 22.

Spontaneous membranoproliferative glomerulonephritis in a young Crl:CD-1(ICR) mouse

Tomoaki Tochitani  1 , Mami Kouchi  1 , Yuta Fuji  1 , Yuka Yoshino  1 , Izumi Matsumoto  1
Affiliations
Case Reports

Spontaneous membranoproliferative glomerulonephritis in a young Crl:CD-1(ICR) mouse

Tomoaki Tochitani et al. J Toxicol Pathol. 2020 Jul.

Abstract

Here, we reported a spontaneous case of membranoproliferative glomerulonephritis observed in a young ICR mouse. A 5-week-old female mouse was euthanized owing to abdominal swelling and increased body weight. At necropsy, generalized subcutaneous edema, and clear, colorless, non-viscous ascites were observed. Histologically, the kidneys showed diffuse, bilateral glomerular lesions. The lesions were characterized by thickening and double contour of the basement membrane and an increase in mesangial cells and matrix, resulting in the narrowing of the capillary lumen. Additionally, eosinophilic hyaloid material accumulated in the subendothelial areas and Bowman's space. The material was positive for periodic acid-Schiff, complement component C3, or immunoglobulin G, stained red by Masson's trichrome, and stained blue by phosphotungstic acid-hematoxylin stain and was considered to be plasma due to glomerular leakage. The glomerular lesion was diagnosed as membranoproliferative glomerulonephritis, and an uncertain endothelial injury was suspected as the cause.

Keywords: CrlCD-1(ICR) mouse; membranoproliferative glomerulonephritis; spontaneous lesion.

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Conflict of interest statement

Authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Fig. 1.
Fig. 1.
HE- and special-stain images of the glomeruli. (A) Capillary walls thicken and the mesangial area is expanded resulting in narrowing of the capillary lumen. Within the Bowman’s space, eosinophilic hyaloid material is accumulated (arrow). Also, in the Bowman’s capsule, swelling or basophilia of parietal epithelial cells can be observed. HE. (B) The thickened capillary tufts and expanded mesangial areas are positive for periodic acid-Schiff (PAS). (C) The collapse of tufts, thickening and double contour of the basement membrane (arrowheads) and increase of mesangial cells and matrix can be seen. The hyaloid material is negative for periodic acid-methenamine-silver (PAM) and observed in subendothelial areas (arrow). PAM. (D) The hyaloid material in subendothelial areas (arrows) and Bowman’s space (arrowhead) is stained red by Masson’s trichrome (MTC).
Fig. 2.
Fig. 2.
Immunohistochemistry for CD31 (A), smooth muscle actin (SMA; B), IgG (C), and C3 (D). Capillary lumen is narrowed, while SMA expression is increased in the mesangial and subendothelial areas. The material accumulated in the Bowman’s space, as well as plasma in the capillary lumen, is positive for IgG, while the capillary walls are negative. On the other hand, the material in both the Bowman’s space and subendothelial areas (arrowheads) is positive for C3.
Fig. 3.
Fig. 3.
Electron microscopy of the glomeruli. (A) Electron dense material can be observed along the capillary wall (arrow). Bar=50 μm. (B) Higher magnification of (A). The electron dense material can be observed in the subendothelial area. Relatively electron-lucent, irregularly-shaped material can also be seen (arrow). The basement membrane is indicated by the arrowheads. Bar=5 μm.
See this image and copyright information in PMC

References

    1. Frazier KS, Seely JC, Hard GC, Betton G, Burnett R, Nakatsuji S, Nishikawa A, Durchfeld-Meyer B, and Bube A. Proliferative and nonproliferative lesions of the rat and mouse urinary system. Toxicol Pathol. 40(4 Supple): 14S–86S. 2012. - PubMed
    1. Kouchi M, Toyosawa K, Matsumoto I, Michimae Y, Tochitani T, Koujitani T, Okimoto K, Funabashi H, and Seki T. Hyaline glomerulopathy with tubulo-fibrillary deposits in young ddY mice. Toxicol Pathol. 39: 975–979. 2011. - PubMed
    1. Masani N, Jhaveri KD, and Fishbane S. Update on membranoproliferative GN. Clin J Am Soc Nephrol. 9: 600–608. 2014. - PMC - PubMed
    1. Raparia K, Usman I, and Kanwar YS. Renal morphologic lesions reminiscent of diabetic nephropathy. Arch Pathol Lab Med. 137: 351–359. 2013. - PubMed
    1. Matsui K, Sato H, Yokoyama Y, Egawa M, and Ito T. Nephrotic syndrome caused by thrombotic microangiopathy during bevacizumab treatment for lung metastases after rectal cancer surgery: A case report. Shimane J Med Sci. 35: 21–25. 2018.

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