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. 1988 Feb;170(2):1021-5.
doi: 10.1128/jb.170.2.1021-1025.1988.

Cloning of the Escherichia coli K-12 hemB gene

J M Li  1 , H Umanoff, R Proenca, C S Russell, S D Cosloy
Affiliations

Cloning of the Escherichia coli K-12 hemB gene

J M Li et al. J Bacteriol. 1988 Feb.

Abstract

An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB.

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