Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020:2163:155-162.
doi: 10.1007/978-1-0716-0696-4_12.

Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for the Detection of Allergen-Specific IgE in Patient Sera Using Allergen Microarrays

Marina Kalli  1   2 , Marcos J C Alcocer  2 , Andrew J Blok  1 , Franco H Falcone  3
Affiliations

Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for the Detection of Allergen-Specific IgE in Patient Sera Using Allergen Microarrays

Marina Kalli et al. Methods Mol Biol. 2020.

Abstract

The presence of allergen-specific IgE (sIgE) in human sera can be determined by measuring the binding of sIgE to solid phase-bound preparations containing the allergens to be tested. These can be complex extracts, purified or recombinant allergens, or peptides. Older methods, such as the IgE CAP test, only allow sIgE measurements to multiple allergens in individual measurements. Newer technologies such as the ImmunoCAP® ISAC test allows semiquantitative testing of sIgE to over a hundred allergens on a protein array. Allergen arrays have higher numerical power, allowing testing to many allergens at the same time, using only a small amount of serum. We have previously demonstrated how allergen arrays can be used in combination with purified peripheral blood basophils, introducing a clinically relevant readout. Here, we describe a protocol and materials that allow the testing of sIgE with multiple allergens in array format, using a humanized fluorescent IgE reporter system (RBL NFAT-DsRed).

Keywords: Allergen; Fluorescent reporter cell line; IgE; Microarrays; NFAT-DsRed; RBL.

PubMed Disclaimer

References

    1. Alessandri C et al (2017) Diagnosing allergic sensitizations in the third millennium: why clinicians should know allergen molecule structures. Clin Transl Allergy 7:21 - DOI - PubMed - PMC
    1. Muraro A et al (2014) EAACI food allergy and anaphylaxis guidelines: diagnosis and management of food allergy. Allergy 69:1008–1025 - DOI - PubMed
    1. Weinberger T, Sicherer S (2018) Current perspectives on tree nut allergy: a review. J Asthma Allergy 11:41–51 - DOI - PubMed - PMC
    1. Carnés J et al (2013) Recently introduced foods as new allergenic sources: sensitisation to Goji berries (Lycium barbarum). Food Chem 137:130–135 - DOI - PubMed
    1. Santos KS et al (2013) Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex. Mol Nutr Food Res 57:1100–1109 - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources