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Observational Study
. 2021 Mar;141(3):678-681.e2.
doi: 10.1016/j.jid.2020.06.030. Epub 2020 Aug 7.

MRGPRX2 Activation Causes Increased Skin Reactivity in Patients with Chronic Spontaneous Urticaria

Maria Shtessel  1 , Nathachit Limjunyawong  2 , Eric T Oliver  3 , Kristin Chichester  3 , Li Gao  3 , Xinzhong Dong  4 , Sarbjit S Saini  3
Affiliations
Observational Study

MRGPRX2 Activation Causes Increased Skin Reactivity in Patients with Chronic Spontaneous Urticaria

Maria Shtessel et al. J Invest Dermatol. 2021 Mar.

Abstract

We present heightened skin reactivity in vivo to two MRGPRX2 drug ligands in patients with chronic spontaneous urticaria. Selectivity for the MRGPRX2 pathway by drug ligands was demonstrated by the loss of drug activation in MRGPRX2-KO LAD2 cells.

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Conflict of interest statement

Conflict of Interest Statement:

None of the authors have any potential financial conflict of interest related to this manuscript.

Figures

Figure 1:
Figure 1:. Skin test responses to MRGPRX2 ligands in healthy and CSU subjects.
Skin test responses to histamine (a) atracurium (b) and icatibant (c). The mean saline wheal in CSU subjects was 1.5 mm ± 2.59 versus 5.53 mm ± 2.74 in healthy controls, p<0.05. Results represent the arithmetic means ±SEM of the wheal size at each concentration. AUC was calculated for each dose response curve (Table S3). Asterisks represent significant dose comparisons (p<0.05)
Figure 2:
Figure 2:. MRGPRX2 ligand induced degranulation of human wild-type and MRGPRX2-deficient LAD2 mast cells.
Atracurium (a) and icatibant (b) degranulation was measured by β-hexosaminidase release. Data represent the means ± SEM of the percent release at each concentration. *, p < 0.05. ***, p < 0.001. The experiments were independently repeated three times.
See this image and copyright information in PMC

References

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