Evaluation of an ELISA for SARS-CoV-2 antibody testing: clinical performances and correlation with plaque reduction neutralization titer

doi:10.1515/cclm-2020-1096

. 2020 Oct 25;58(11):e247-e249.

doi: 10.1515/cclm-2020-1096.

Evaluation of an ELISA for SARS-CoV-2 antibody testing: clinical performances and correlation with plaque reduction neutralization titer

Andrea Padoan^{1

2}, Francesco Bonfante³, Laura Sciacovelli², Chiara Cosma², Daniela Basso^{1

2}, Mario Plebani^{1

2}

Affiliations

¹ Department of Medicine-DIMED, Medical School, University of Padova, Padova, Italy.
² Department of Laboratory Medicine, University-Hospital of Padova, Padova, Italy.
³ Laboratory of Experimental Animal Models, Division of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.

PMID: 32772001
DOI: 10.1515/cclm-2020-1096

Free article

Evaluation of an ELISA for SARS-CoV-2 antibody testing: clinical performances and correlation with plaque reduction neutralization titer

Andrea Padoan et al. Clin Chem Lab Med. 2020.

Free article

. 2020 Oct 25;58(11):e247-e249.

doi: 10.1515/cclm-2020-1096.

Authors

Andrea Padoan^{1

2}, Francesco Bonfante³, Laura Sciacovelli², Chiara Cosma², Daniela Basso^{1

2}, Mario Plebani^{1

2}

Affiliations

¹ Department of Medicine-DIMED, Medical School, University of Padova, Padova, Italy.
² Department of Laboratory Medicine, University-Hospital of Padova, Padova, Italy.
³ Laboratory of Experimental Animal Models, Division of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.

PMID: 32772001
DOI: 10.1515/cclm-2020-1096

No abstract available

Keywords: SARS-CoV-2; antibodies; clinical performances; immunoassays; neutralization; serology.

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References

1. Bohn, MK, Lippi, G, Lippi, G, Horvath, A, Horvath, A, Sethi, S, et al. Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence. Clin Chem Lab Med 2020;58:1037–52. https://doi.org/10.1515/cclm-2020-0722.
1. Lippi, G, Simundic, AM, Plebani, M. Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19). Clin Chem Lab Med 2020;58:1070–6. https://doi.org/10.1515/cclm-2020-0285.
1. Krammer, F, Simon, V. Serology assays to manage COVID-19. Science 2020;368:1060–1. https://doi.org/10.1126/science.abc1227.
1. Plebani, M, Padoan, A, Negrini, D, Carpinteri, B, Sciacovelli, L. Diagnostic performances and thresholds: the key to harmonization in serological SARS-CoV-2 assays?. Clin Chim Acta 2020;509:1–7. https://doi.org/10.1016/j.cca.2020.05.050.
1. Colavita, F, Brogi, A, Lapa, D, Bordi, L, Matusali, G, Meschi, S, et al. Evaluation of ELISA tests for the qualitative determination of IgG IgM and IgA to SARS-CoV-2. Medrxiv 2020. Available from: http://medrxiv.org/cgi/content/short/2020.05.24.20111682.

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[1] Bohn, MK, Lippi, G, Lippi, G, Horvath, A, Horvath, A, Sethi, S, et al. Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence. Clin Chem Lab Med 2020;58:1037–52. https://doi.org/10.1515/cclm-2020-0722.

[2] Bohn, MK, Lippi, G, Lippi, G, Horvath, A, Horvath, A, Sethi, S, et al. Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence. Clin Chem Lab Med 2020;58:1037–52. https://doi.org/10.1515/cclm-2020-0722.

[3] Lippi, G, Simundic, AM, Plebani, M. Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19). Clin Chem Lab Med 2020;58:1070–6. https://doi.org/10.1515/cclm-2020-0285.

[4] Lippi, G, Simundic, AM, Plebani, M. Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19). Clin Chem Lab Med 2020;58:1070–6. https://doi.org/10.1515/cclm-2020-0285.

[5] Krammer, F, Simon, V. Serology assays to manage COVID-19. Science 2020;368:1060–1. https://doi.org/10.1126/science.abc1227.

[6] Krammer, F, Simon, V. Serology assays to manage COVID-19. Science 2020;368:1060–1. https://doi.org/10.1126/science.abc1227.

[7] Plebani, M, Padoan, A, Negrini, D, Carpinteri, B, Sciacovelli, L. Diagnostic performances and thresholds: the key to harmonization in serological SARS-CoV-2 assays?. Clin Chim Acta 2020;509:1–7. https://doi.org/10.1016/j.cca.2020.05.050.

[8] Plebani, M, Padoan, A, Negrini, D, Carpinteri, B, Sciacovelli, L. Diagnostic performances and thresholds: the key to harmonization in serological SARS-CoV-2 assays?. Clin Chim Acta 2020;509:1–7. https://doi.org/10.1016/j.cca.2020.05.050.

[9] Colavita, F, Brogi, A, Lapa, D, Bordi, L, Matusali, G, Meschi, S, et al. Evaluation of ELISA tests for the qualitative determination of IgG IgM and IgA to SARS-CoV-2. Medrxiv 2020. Available from: http://medrxiv.org/cgi/content/short/2020.05.24.20111682.

[10] Colavita, F, Brogi, A, Lapa, D, Bordi, L, Matusali, G, Meschi, S, et al. Evaluation of ELISA tests for the qualitative determination of IgG IgM and IgA to SARS-CoV-2. Medrxiv 2020. Available from: http://medrxiv.org/cgi/content/short/2020.05.24.20111682.

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Evaluation of an ELISA for SARS-CoV-2 antibody testing: clinical performances and correlation with plaque reduction neutralization titer

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Evaluation of an ELISA for SARS-CoV-2 antibody testing: clinical performances and correlation with plaque reduction neutralization titer

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