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. 2021 Feb;19(2):215-217.
doi: 10.1111/pbi.13463. Epub 2020 Sep 16.

Flowering Locus T chimeric protein induces floral precocity in edible citrus

Judith P Sinn  1 , Jeremy B Held  1   2 , Chad Vosburg  1 , Sara M Klee  1 , Vladimir Orbovic  3 , Earl L Taylor  4 , Tim R Gottwald  4 , Ed Stover  4 , Gloria A Moore  5 , Timothy W McNellis  1
Affiliations

Flowering Locus T chimeric protein induces floral precocity in edible citrus

Judith P Sinn et al. Plant Biotechnol J. 2021 Feb.
No abstract available

Keywords: accelerated breeding; blooming; early flowering; grapefruit; juvenility.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Chimeric PtFT1 fusion protein expression in transgenic grapefruit produces blooming precocity. (a) PtFT1‐scFv constitutive expression cassette. (b) Detection of 47.9 kilodalton (kD) PtFT1‐scFv protein in shoot tip extracts using anti‐cMyc immunoblotting; ‘Duncan’, untransformed grapefruit; and protein loading normalized to tissue weight. (c) Growth form of low‐ (line L), moderate‐ (line J) and high‐expressor (lines H and O) plants. (d) Thorniness in transgenic control (line E), low (line L) and high expressors (lines O and H). (e) Phenotype summary. (f) Precocious flowering in a strongly precocious line. (g) Flower morphology in a strongly precocious line. (h) Germinated pollen grains. (i) Seed set in an immature fruit from a hand‐pollinated flower at 5 months after pollination. (j) Ripe fruit from an unpollinated flower. (k) Flower (arrow) on rooted clone of a mildly precocious line. (l,m) Precious phenotypes of ‘Carrizo’ rootstock and ‘Jackson’ hybrid grapefruit PtFT1‐scFv transformants. Arrows in (m) indicate flower buds. [Colour figure can be viewed at wileyonlinelibrary.com]
See this image and copyright information in PMC

References

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